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由来生物
mouse
品質水準
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
B7, monoclonal
交差性
human
メーカー／製品名
Chemicon®
濃度
2.4 mg/mL
テクニック
dot blot: suitable
immunohistochemistry: suitable
アイソタイプ
IgG1κ
適合性
not suitable for Western blot
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... CMA1(1215)
詳細
Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.
特異性
Reacts with human mast cell chymase.
免疫原
Chymase purified from human skin.
アプリケーション
Research Category
炎症及び免疫
炎症及び免疫
Research Sub Category
炎症及び自己免疫疾患
炎症及び自己免疫疾患
Anti-Chymase Antibody, clone B7 is an antibody against Chymase for use in DB, IH.
Dot blotting: 0.5-1.0 μg/mL.
Immunohistochemistry: 0.5-1.0 μg/mL. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.
Not recommended for use on Western blots.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 0.5-1.0 μg/mL. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.
Not recommended for use on Western blots.
Optimal working dilutions must be determined by end user.
物理的形状
Format: Purified
Purified by protein G affinity chromatography. Liquid in PBS with 20% glycerol, pH 7.4.
保管および安定性
Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. Antibody may also be stored at -20ºC in undiluted aliquots.
アナリシスノート
Control
POSITIVE CONTROL: lung, heart, skin, placenta.
POSITIVE CONTROL: lung, heart, skin, placenta.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB1254:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Co-cultivation of mast cells and Fc epsilon RI alpha+ dendritic-like cells from human hip bone marrow.
D Kaur, P Berger, S M Duffy, C E Brightling, P Bradding
Clinical and Experimental Allergy null
M Yamada et al.
Kidney international, 59(4), 1374-1381 (2001-03-22)
Mast cells (MCs) are known to participate in various types of chronic disease, but their role in chronic renal rejection is poorly understood. Recently, distinct phenotypes of MCs have been described in humans by the demonstration of one protease, chymase.
Buffy coat preparation is a convenient source of progenitors for culturing mature human mast cells.
X S Wang, K H Yip, S W Sam, H Y A Lau
Journal of Immunological Methods null
Mast cell regulation of human endometrial matrix metalloproteinases: A mechanism underlying menstruation.
J Zhang, G Nie, W Jian, D E Woolley, L A Salamonsen
Biology of Reproduction null
Fixation with Carnoy's fluid reduces the number of chymase-positive mast cells: not all chymase-positive mast cells are also positive for tryptase
KleinJan, A. et al.
Allergy, 51, 614-620 (1996)
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