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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
クローン
G3, monoclonal
交差性
human
メーカー／製品名
Chemicon®
テクニック
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
詳細
Tryptase is the most abundant secretory granule-derived serine proteinase contained in mast cells. Tryptase has recently been used as a marker for mast cell activation and is involved with allergic response. Tryptase may act as a mitogen for fibroblast lines. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis.
特異性
Recognizes Mast cell tryptase. Will show reactivity to basophils but to a lesser degree.
アプリケーション
Research Category
炎症及び免疫
炎症及び免疫
Research Sub Category
炎症及び自己免疫疾患
炎症及び自己免疫疾患
Flow Cytometry Analysis: A previous lot of this antibody has been used on fixed cells in FACS using a conjugated secondary antibody. (Sperr, W., et al., 2001).
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
This Anti-Tryptase Antibody, Mast Cell, clone G3 is validated for use in FC, IH, WB for the detection of Tryptase.
品質
Evaluated by western blot on human fetal skin lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected tryptase in 10 µg of human fetal skin lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected tryptase in 10 µg of human fetal skin lysate.
ターゲットの説明
31 kDa
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
(see product datasheet for specific buffer formulation)
(see product datasheet for specific buffer formulation)
保管および安定性
Maintain at 2–8°C for the duration listed on the product datasheet. (See product datasheet for storage conditions)
アナリシスノート
Control
Mast cells, basophils
Human fetal skin lysate
Mast cells, basophils
Human fetal skin lysate
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB1222:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
A human lung tumor microenvironment interactome identifies clinically relevant cell-type cross-talk.
Andrew J Gentles et al.
Genome biology, 21(1), 107-107 (2020-05-10)
Tumors comprise a complex microenvironment of interacting malignant and stromal cell types. Much of our understanding of the tumor microenvironment comes from in vitro studies isolating the interactions between malignant cells and a single stromal cell type, often along a
T Kinoshita et al.
Blood, 94(2), 496-508 (1999-07-09)
In the present study, we attempted to clarify the effects of interleukin-6 (IL-6) on the growth and properties of human mast cells using cultured mast cells selectively generated by stem cell factor (SCF) from CD34(+) cord blood cells. The addition
Regulation of matrix metalloproteinases in human endometrium.
L A Salamonsen, J Zhang, A Hampton, L Lathbury, L A Salamonsen, J Zhang, A Hampton, L Lathbury
Human Reproduction null
Christian Breuer et al.
World journal of gastroenterology, 16(34), 4363-4366 (2010-09-08)
Constipation and fecal impaction are frequent and distressing complaints in pediatric gastroenterology. Especially in neurologically handicapped children, treatment of severe forms of slow-transit constipation (STC) can be difficult. In the majority of cases, STC is of unknown etiology. However, in
Serum inhibitors for human mast cell growth: possible role of retinol.
S Ishida, T Kinoshita, N Sugawara, T Yamashita, K Koike
Allergy null
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