MAB1233-I

Anti-Estradiol (6-BSA) Antibody

purified antibody, clone 6E1, from mouse

• UNSPSCコード: 12352203
• NACRES: NA.41
• クローン: 6E1, monoclonal
• application: ELISA; ICC; activity assay
• 化学種の反応性: human
• テクニック: ELISA: suitable; activity assay: suitable; immunocytochemistry: suitable

特性

• 由来生物: mouse
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 6E1, monoclonal
• 化学種の反応性: human
• 包装: antibody small pack of 100 μL
• テクニック: ELISA: suitable; activity assay: suitable; immunocytochemistry: suitable
• アイソタイプ: IgG
• 輸送温度: dry ice
• 保管温度: -10 to -25°C
• ターゲットの翻訳後修飾: unmodified

詳細

特異性

Clone 9E1 is a mouse monoclonal antibody that detects BSA-conjugated Estradiol.

免疫原

BSA-conjugate Estradiol (Estradiol-6-BSA).

アプリケーション

Quality Control TestingEvaluated by Immunocytochemistry in OVCAR 3 cells.Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected Estradiol (6-BSA) in OVCAR 3 cells.Tested ApplicationsELISA Analysis: Various titrations (2-fold serial dilutions) starting at 100 ng/mL from a representative lot detected Estradiol (6-BSA).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Estradiol (6-BSA), clone 6E1, Cat. No. MAB1233-I, is a mouse monoclonal antibody that detects Estradiol-6-BSA and is tested for use in ELISA and Immunocytochemistry.

ターゲットの説明

Estradiol is a major female sex hormone that regulates estrous and menstrual cycle. It is also present in smaller quantities in males where it is an active metabolic product of testosterone. In females, it is mainly produced by the granulosa cells in the follicles of ovaries. Smaller amounts of estradiol are also produced by the adrenal glands. It acts as an agonist for the estrogen receptor (ER) and can bind to both ERa and ERb. It is synthesized from cholesterol where androstenedione is a key intermediate which is converted to estradiol by aromatase. It is metabolized via hydroxylation into catechol estrogens. In the liver it can undergo hydroxylation into 2-hydoxyestradiol by CYP1A2, CYP3A4, and CYP2C9. Estradiol is reported to directly act on the central nervous system to modulate energy balance and reduced levels of estradiol following ovarian insufficiency have been linked to menopause, hyperphagia, and weight gain. (Ref.: Gonzales-Garcia, I., et al. (2018). Cell Rep. 25(2): 413-423).

物理的形状

Purified mouse monoclonal antibody IgG in PBS without azide.

保管および安定性

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable