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由来生物
mouse
品質水準
アッセイ
>95% (SDS-PAGE)
メーカー／製品名
Chemicon®
テクニック
cell culture | stem cell: suitable
不純物
<0.1 ng/mg endotoxin (of LIF)
入力
sample type mesenchymal stem cell(s)
sample type neural stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
NCBIアクセッション番号
UniProtアクセッション番号
詳細
Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Mouse LIF is a 22 kDa protein containing 203 amino acid residues.
mLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.
アプリケーション
5 μg, mouse recombinant LIF protein, expressed in E. coli, suitable for stem cell culture
RECOMMENDED QC PROTOCOL
M1 Bioassay
1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.
2. Add 100 μL of sample or mLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.
3. Add 100 μL of 5% FCS in isotonic saline to two control slides.
4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.
5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).
Visit www.esgro-lif.com for additional information
Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.
M1 Bioassay
1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.
2. Add 100 μL of sample or mLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.
3. Add 100 μL of 5% FCS in isotonic saline to two control slides.
4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.
5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).
Visit www.esgro-lif.com for additional information
Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.
単位の定義
The specific activity is ≥1 x 10(E8) units/mg, where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.
物理的形状
Liquid in PBS, pH 7.4 and 0.02% Tween 20. No preservativies added.
保管および安定性
Maintain at 2-8°C for 6 - 12 months. Further dilutions should be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added.
Manufactured by CHEMICON International, Inc. LIF is protected under US Patent
Manufactured by CHEMICON International, Inc. LIF is protected under US Patent
アナリシスノート
Tested negative in both aseptic and microplasmic tests.
The activity of mouse LIF is determined by the ability to induce differentiation of murine M1 myeloid leukemic cells. The minimum detectable concentration of mouse LIF in this assay is 0.5 ng/mL.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
LIF2005-K:
LIF2010-K:
試験成績書（COA）
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