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Key Documents

安全性情報

IM09L

Sigma-Aldrich

Anti-MMP-9 (Ab-1) Mouse mAb (6-6B)

lyophilized, clone 6-6B, Calbiochem®

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

mouse

品質水準

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

6-6B, monoclonal

形状

lyophilized

含まれません

preservative

化学種の反応性

human

メーカー/製品名

Calbiochem®

保管条件

OK to freeze

アイソタイプ

IgG1

輸送温度

ambient

保管温度

2-8°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... MMP9(4318)

詳細

Matrix metalloproteinases (MMP′s) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMP′s share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis.MMP-9 (Gelatinase B, 92 kDa gelatinase/type IV collagenase) is secreted as a 92 kDa zymogen which is proteolytically processed to the 83 kDa active form; a 68 kDa has also been detected. MMP-9, along with its most closely related member of the MMP family, MMP-2, show substrate specificity toward type IV and V collagens, gelatin and elastin. Numerous studies have shown a correlation between collagenase expression and metastatic potential. Elevated levels of MMP-9 in plasma suggest that it may be a useful marker for the diagnosis or prognosis of cancer in general.
Purified mouse monoclonal antibody. Recognizes the ~92 kDa latent and the ~83 kDa active forms of human MMP-9 under non-reducing conditions, but only the latent form under reducing conditions.
Recognizes the ~92 kDa latent and the ~83 kDa active forms of MMP-9 under non-reducing conditions, but only the latent form under reducing conditions. Inhibits the enzymatic activity of MMP-9. For paraffin sections, use Cat. No. IM37L.
This Anti-MMP-9 (Ab-1) Mouse mAb (6-6B) is validated for use in Immunoblotting, Immunoprecipitation, Neutralization Studies, Paraffin Sections for the detection of MMP-9 (Ab-1).

免疫原

Human
MMP-9 from conditioned medium of PMA-stimulated HT-1080 human fibrosarcoma cells

アプリケーション

Immunoblotting (2 µg/ml)

Immunoprecipitation (see appplication references)

Neutralization Studies (see comments and application references)

Paraffin Sections (not recommended)

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from a volatile buffer, 100 µg BSA.

再構成

Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Resuspend the antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Freezing of aliquots is best for long-term storage of reconstituted product; repetitive freezing and thawing should be avoided.

アナリシスノート

Positive Control
MMP-9 protein (Cat. Nos. PF024 or PF038)

その他情報

Cottam, D.W. and Rees, R.C. 1993. Intl. J. of Oncol.2, 861.
Nakajima, M., et al. 1993. Cancer Res.53, 5802.
Ramos-DeSimone, et al. 1993. Hybridoma. 12, 349.
Stetler-Stevenson, W.G., et al. 1993. FASEB.7, 1434.
Zucker, S., et al. 1993. Cancer Res.53, 140.
Woessner, J.F. 1991. FASEB. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1; 99-106.
Inhibits MMP-9 enzymatic activity. For staining paraffin sections, use Anti-MMP-9 (626-644) (Ab-3) Mouse mAb (56-2A4) (Cat. No. IM37L). Antibody should be titrated for optimal results in individual systems.

法的情報

Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 2


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

IM09L-20UG:
IM09L-100UG:
IM09L-3MG:
IM09L-1MG:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Yao Tong et al.
Journal of inflammation research, 14, 5079-5094 (2021-10-23)
Acute lung injury (ALI) is a severe respiratory disease with high rates of morbidity and mortality. Many mediators regarding endogenous or exogenous are involved in the pathophysiology of ALI. Here, we have uncovered the involvement of integrins and matrix metalloproteinases
Min Hee Park et al.
Stem cells (Dayton, Ohio), 34(8), 2145-2156 (2016-04-20)
Hematopoietic stem/progenitor cell (HSPC) mobilization is an essential homeostatic process regulated by the interaction of cellular and molecular components in bone marrow niches. It has been shown by others that neurotransmitters released from the sympathetic nervous system regulate HSPC egress
Daniel J Price et al.
Cell communication & adhesion, 9(2), 87-102 (2002-12-19)
We studied the invasion of HMT-3522 breast epithelial cells in response to epidermal growth factor (EGF), and the associated signaling pathways. HMT-3522 T4-2 cells were shown to invade Matrigel-coated Transwell membranes in response to EGF while HMT-3522 S-1 cells failed
Andreas Behren et al.
Cancer research, 65(24), 11613-11621 (2005-12-17)
Papillomaviruses are involved in the development of cancers of the female cervix, head and neck, and skin. An excellent model to study papillomavirus-induced tumor induction and progression is the New Zealand White rabbit, where the skin is infected with the
Agnes Schäfer et al.
Frontiers in oncology, 12, 826273-826273 (2022-04-05)
Glioblastoma (GBM) as the most common and aggressive brain tumor is characterized by genetic heterogeneity, invasiveness, radio-/chemoresistance, and occurrence of GBM stem-like cells. The metalloprotease-disintegrin ADAM8 is highly expressed in GBM tumor and immune cells and correlates with poor survival.

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