GR13L

Anti-EGFR (Ab-3) Mouse mAb (225)

lyophilized, clone 225, Calbiochem^®

• 別名: Anti-EGF-R
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 225, monoclonal
• 形状: lyophilized
• 含まれません: preservative
• 化学種の反応性: human
• 以下との反応禁止: rat, mouse
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• アイソタイプ: IgG1
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... EGFR(1956) ; mouse ... Egfr(13649) ; rat ... Egf(25313)

詳細

詳細

Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.

Recognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells.

免疫原

Human

partially purified, human EGFR from A431 cells

アプリケーション

Immunoprecipitation (1-2 µg per sample)

Neutralization Studies (2 µg/ml, see comments and application references)

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.

再構成

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.

アナリシスノート

Negative Control
HL-60 cells

Positive Control
A431 cells

その他情報

Hunter T. 1984. Nature311, 414.
Reynolds, F.H., et. al 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable