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Merck

F1903

Sigma-Aldrich

Fibronectin 40 kDa α Chymotryptic Fragment (Heparin-binding region), Human purified

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About This Item

UNSPSCコード:
12352202
eCl@ss:
32160405
NACRES:
NA.75

由来生物

human

品質水準

形状

lyophilized

分子量

40 kDa

メーカー/製品名

Chemicon®

濃度

1 mg/mL

テクニック

cell culture | mammalian: suitable

入力

sample type epithelial cells
sample type: human embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

遺伝子情報

human ... FN1(2335)

詳細

Heparin-binding 40 kDa fragment of human plasma fibronectin purified from chymotryptic digest of human plasma fibronectin. This preparation contains the CS-1 heparin-binding region of fibronectin. Heparin-binding fragment promotes cytoskeleton organization in integrin signaling. This preparation shows one major band at 40 kDa in SDS-PAGE with no higher molecular weight species present. Smaller proteolytic fragments may be present.

Human sourced raw material used in the production of this product tested negative for hepatitis B virus, hepatitis C virus (HCV), human immunodeficiency virus type-1 (HIV-1) and type-2 (HIV-2), human T-cell lymphotropic virus (HTLV-1 and HTLV-2) and Treponema pallidum. Handle as if potentially infectious.
Product Source: Human

アプリケーション

ELISA

Affinity chromatography

Possibly for molecular mapping of fibronectin function

Cell attachment and integrin signaling assays

Optimal working dilutions must be determined by the end user.

物理的形状

Purified by gelatin-Sepharose and heparin-Sepharose column chromatography. Lyophilized from sodium citrate buffered saline, 20mM, NaCl 150mM, pH 7.4. Reconstitute with distilled water.

保管および安定性

Maintain lyophilized material at -20°C for up to one year from date of receipt. After reconstitution, maintain at -20°C for up to six months in undiluted aliquots. Avoid repeated freeze/thaw cycles.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

保管分類コード

11 - Combustible Solids

WGK

WGK 1


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

F1903:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Alignment of biologically active domains in the fibronectin molecule
Ruoslahti, E. et al.
The Journal of Biological Chemistry, 256, 7277-7281 (1981)
Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule.
Pierschbacher, M D, et al.
Cell, 26, 259-267 (1981)

資料

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

プロトコル

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs at sigmaaldrich.com

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

関連コンテンツ

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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