BI0166

Activator Reagent 0.3M BMT in AcN

Biosynthesis Novabiochem^®

• UNSPSCコード: 41116006

特性

• 品質水準: 200
• 製品種目: Novabiochem^®
• 形状: liquid
• 反応適合性: reaction type: Coupling Reactions; reagent type: catalyst
• 転移温度: flash point 2 °C (Acetonitrile)
• 輸送温度: ambient
• 保管温度: 2-30°C

詳細

詳細

Activator Reagent 0.3M 5-benzylmercaptotetrazole (BMT) in acetonitrile (AcN) is also known as benzylthiotetrazole (BTT) or benzyl-1H-tetrazole). It is used as a phosphoramidite activator reagent for oligonucleotide synthesis.

アプリケーション

0.3M BMT in AcN can be used as a reagent in solid phase phosphorylation of the oligomers. The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the 5′hydroxyl group of the growing oligonucleotide chain. The high activation potential of this reagent may be due to its greater acidity (pKa=4.08), which makes it easier to protonate the trivalent phosphorus in the initial stage of activation and increase the speed of the coupling reaction.

特徴および利点

• efficient and rapid activation reagent that produces less
• phosphoramidite waste      
• higher solubility at lower temperatures      
• does not crystallize or clog lines       
• safer reagent to use

アナリシスノート

Color (APHA): 10APHA max
Component: Acetonitrile Purity (By GC): 99.9% min
Form: Clear Liquid
Identity (IR-spectrum): Conforms
Molarity of 5-Benzylmercaptotetrazole: 0.28-0.32M
Water (H2O): 1-30ppm

法的情報

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS02,GHS07
• シグナルワード: Danger
• 危険有害性情報: H225 - H302 + H312 + H332 - H319
• 注意書き: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 3
• 引火点(°F): 35.6 °F - (Acetonitrile)
• 引火点(℃): 2 °C - (Acetonitrile)