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詳細
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
特異性
Specific for mouse IgG, heavy and light chain.
アプリケーション
Research Category
二次抗体及びコントロール抗体
二次抗体及びコントロール抗体
Research Sub Category
二重標識用 吸収処理済み二次抗体
二重標識用 吸収処理済み二次抗体
Donkey anti-Mouse IgG Antibody, biotin conjugate, Species Adsorbed detects level of Donkey Mouse IgG & has been published & validated for use in ELISA & WB.
EIA and Western blots: 1:5,000-1:100,000.
Immunohistochemistry: 1:500-1:5,000.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 1:500-1:5,000.
Optimal working dilutions must be determined by end user.
物理的形状
Immunoaffinity Purified and Immunodepletion
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25M NaCl, pH 7.6 with 15 mg/mL BSA and 0.01% thimerosal.
RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.
RECONSTITUTION:
Reconstitute with 500 μL of sterile distilled water.
保管および安定性
Maintain lyophilized product at 2–8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
11 - Combustible Solids
WGK
WGK 3
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Patterns of axonal collateralization of single layer V cortical projection neurons in the rat presubiculum.
Honda Y, Furuta T, Kaneko T, Shibata H, Sasaki H
The Journal of Comparative Neurology null
Juan Turati et al.
Journal of neurochemistry, 161(4), 366-382 (2022-04-13)
Subtype 3 metabotropic glutamate receptor (mGlu3R) displays a broad range of neuroprotective effects. We previously demonstrated that mGlu3R activation in astrocytes protects hippocampal neurons from Aβ neurotoxicity through stimulation of both neurotrophin release and Aβ uptake. Alternative-spliced variants of mGlu3R
Takaki Yahiro et al.
Scientific reports, 7(1), 5031-5031 (2017-07-12)
Thermoregulatory behaviour, such as migration to a comfortable thermal environment, is a representative innate animal behaviour and facilitates effective autonomic regulation of body temperature with a reduced cost of resources. Here we determine the central thermosensory ascending pathway that transmits
Eriko Kuramoto et al.
The European journal of neuroscience, 33(1), 95-109 (2010-11-16)
Motor thalamic nuclei, ventral anterior (VA), ventral lateral (VL) and ventral medial (VM) nuclei, receive massive glutamatergic and GABAergic afferents from the cerebellum and basal ganglia, respectively. In the present study, these afferents were characterized with immunoreactivities for glutamic acid
G M Reynolds et al.
Journal of clinical pathology, 48(12), 1138-1140 (1995-12-01)
This report describes the characterisation of a polyclonal sheep antiserum against the Ki67 antigen. On western blots, this antiserum recognises a pair of bands of high molecular weight identical with those seen with another polyclonal Ki67 antiserum and the MIB
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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