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Key Documents

安全性情報

AP1063

Sigma-Aldrich

PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb

liquid, Calbiochem®

別名:

Anti-Pyruvate Dehydogenase pSer²³² Rabbit pAb

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

rabbit

品質水準

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

primary antibodies

クローン

polyclonal

形状

liquid

含みます

≤0.1% sodium azide as preservative

化学種の反応性

mouse, human, rat

メーカー/製品名

Calbiochem®

保管条件

OK to freeze
avoid repeated freeze/thaw cycles

輸送温度

wet ice

保管温度

−20°C

ターゲットの翻訳後修飾

phosphorylation (pSer232)

詳細

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232.
Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser232 in HEK293 cells.
This PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb is validated for use in Immunoblotting, IF, IP for the detection of PDH-E1α (pSer²³²).

免疫原

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³² phosphorylation site of human PDH-E1α

アプリケーション



Immunoblotting (0.25 g/ml)
Immunofluorescence (Please see comments)
Immunoprecipitation (1 g/ml)

警告

Toxicity: Standard Handling (A)

物理的形状

In PBS.

再構成

Following initial thaw, aliquot and freeze (-20°C).

アナリシスノート

Positive Control
HEK293 cells

その他情報

Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
This antibody has been known to work with immunofluorescence. Antibody should be titrated for optimal results in individual systems.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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保管分類コード

12 - Non Combustible Liquids

WGK

nwg

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

AP1063-5MG:
AP1063-50UG:
AP1063-UG:
AP1063-1MG:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Lærke Bertholdt et al.
Journal of applied physiology (Bethesda, Md. : 1985), 124(3), 729-740 (2017-12-02)
Recruitment of fatty acids from adipose tissue is increased during fasting. However, the molecular mechanisms behind fasting-induced metabolic regulation in human adipose tissue and the potential impact of training state in this are unknown. Therefore the aim of the present
Shelby L Oke et al.
International journal of molecular sciences, 22(14) (2021-07-25)
The rates of gestational cannabis use have increased despite limited evidence for its safety in fetal life. Recent animal studies demonstrate that prenatal exposure to Δ9-tetrahydrocannabinol (Δ9-THC, the psychoactive component of cannabis) promotes intrauterine growth restriction (IUGR), culminating in postnatal
Richard A Harris et al.
eNeuro, 6(1) (2019-02-28)
The consolidation of newly formed memories and their retrieval are energetically demanding processes. Aerobic glycolysis (AG), also known as the Warburg effect, consists of the production of lactate from glucose in the presence of oxygen. The astrocyte neuron lactate shuttle
Arpit Sharma et al.
eLife, 8 (2019-07-16)
Metabolic cycles are a fundamental element of cellular and organismal function. Among the most critical in higher organisms is the Cori Cycle, the systemic cycling between lactate and glucose. Here, skeletal muscle-specific Mitochondrial Pyruvate Carrier (MPC) deletion in mice diverted
Rosa Ferriero et al.
Science translational medicine, 5(175), 175ra31-175ra31 (2013-03-08)
Lactic acidosis is a buildup of lactic acid in the blood and tissues, which can be due to several inborn errors of metabolism as well as nongenetic conditions. Deficiency of pyruvate dehydrogenase complex (PDHC) is the most common genetic disorder

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