ABT168

Anti-Girdin Antibody

from rabbit, purified by affinity chromatography

• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human, monkey
• 化学種の反応性（ホモロジーによる予測）: rat (immunogen homology)
• テクニック: immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• NCBIアクセッション番号: XP_223709
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CCDC88A(55704)

詳細

詳細

Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.

特異性

Other homologies: Mouse (97% sequence homology).

This antibody recognizes the coiled-coil domain of Girdin.

免疫原

Epitope: Coiled coil domain.

GST-tagged recombinant protein corresponding to the coiled coil domain rat Girdin.

アプリケーション

Research Sub Category
細胞骨格

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Girdin in human colorectal cancer tissues.

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Girdin in HeLa, Cos-7, and Att20 cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in HeLa cell lysate.

Research Category
Cell Structure

This Girdin antibody is validated for use in WB, IHC, IF & IP for the detection of the Girdin protein.

品質

Evaluated by Western Blotting in Cos-7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Girdin in 10 µg of Cos-7 cell lysate.

ターゲットの説明

~220 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Cos-7 cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable