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Key Documents

安全性情報

ABT1358M

Sigma-Aldrich

Anti-Versican Antibody, Alpha Domain

serum, from rabbit

別名:

Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding protein, GHAP, Large fibroblast proteoglycan, PG-M

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

rabbit

品質水準

抗体製品の状態

serum

抗体製品タイプ

primary antibodies

クローン

polyclonal

化学種の反応性

rat

包装

antibody small pack of 25 μL

テクニック

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
radioimmunoassay: suitable
western blot: suitable

アイソタイプ

IgG

NCBIアクセッション番号

UniProtアクセッション番号

ターゲットの翻訳後修飾

unmodified

遺伝子情報

詳細

Versican core protein (UniProt: Q9ERB4; also known as Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding protein, GHAP, Large fibroblast proteoglycan, PG-M) is encoded by the Vcan (also known as Cspg2) gene (Gene ID: 114122) in rat. Versican is a large multi-domain chondroitin sulphate proteoglycan that is secreted by fibroblasts and other types of cells. Versican is present in most of the retina including the outer nuclear layer, the outer plexiform layer, and the inner plexiform layer. It is synthesized with a 20 amino acid signal peptide, which is subsequently cleaved. Veriscan plays a role in intercellular signaling and in connecting cells with the extracellular matrix and is also involved in axon regeneration, central nervous system and skeletal muscle development, and cell adhesion. It has unique N- and C-terminal globular regions, each with multiple motifs. It contains one C-type lectin domain, two EGF-like domains, one Ig-like V-type domain, two link domains and one Sushi (CCP/SCR) domains. It also has a N-terminal hyaluronic acid (HA) binding domain that has homology with the G1 and G2 domains of aggrecan. Four isoforms of versican termed as V0, V1, V2, and V3, have been reported with molecular weights of 370, 263, 180, and 74 kDa, respectively. Each isoform contains different lengths of the GAG binding region. V0 contains two GAG binding regions called CSalpha and CSbeta domains. V1 isoform has only CSbeta and V2 isoform has only CSalpha domain. V3 isoform contains only G1 and G3 domains and lacks GAG attachment sites. Defects in Vcan gene result in Wagner syndrome type 1, a type of vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a pre-retinal avascular membrane.

特異性

This rabbit polyclonal antibody detects versican in rat eye sections.

免疫原

Epitope: unknown
GST-tagged recombinant fragment corresponding to the first 321 amino acids of rat Versican.

アプリケーション

Research Category
細胞骨格
Anti-Versican, Alpha Domain, Cat. No. ABT1358, is a rabbit polyclonal antibody that detects versican core protein that has been tested for use in Immunohistochemistry (Paraffin), Radioimmunoassay, and Western Blotting.
Radioimmunoassay Analysis: A representative lot detected Versican, Alpha Domain in Postnatal rat brain (Milev, P., et. al. (1998). Biochem Biopys Res Commun. 247(2):207-12).

Immunohistochemistry Analysis: A representative lot detected Versican, Alpha Domain in Embryonic day 16 rat eye, P0, P9 and adult rat retina (Popp, S., et. al. (2004). Exp Eye Res. 79(3):351-6) and Embryonic day 16, 19 rat brain and postnatal day 7 cerebellum, embryonic day 19 spinal cord (Popp, S., et. al. (2003). Dev Dyn. 227(1):143-9).

Western Blotting Analysis: A representative lot detected Versican, Alpha Domainin in purified proteoglycans from a PBS extract of 7-day rat brain with chondroitinase ABC treatment (Popp, S., et. al. (2003). Dev Dyn. 227(1):143-9).

品質

Evaluated by Immunohistochemistry in rat eye retina tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Versican, Alpha Domain in rat eye retina tissues.

ターゲットの説明

300.01 kDa calculated.

物理的形状

Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

ABT1358:
ABT1358-25UL:


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