ABS1668M

Anti-CHMP3

serum, from rabbit

• 別名: Charged multivesicular body protein 3, Chromatin-modifying protein 3, Neuroendocrine differentiation factor, Vacuolar protein sorting-associated protein 24, hVps24
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: polyclonal
• application: ICC; WB
• 化学種の反応性: human
• テクニック: immunocytochemistry: suitable; western blot: suitable

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: human
• 包装: antibody small pack of 25 μL
• テクニック: immunocytochemistry: suitable; western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_057163
• UniProtアクセッション番号: Q9Y3E7
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CHMP3(51652)

詳細

詳細

Charged multivesicular body protein 3 (UniProt: Q9Y3E7; also known as Chromatin-modifying protein 3, CHMP3, Neuroendocrine differentiation factor, Vacuolar protein sorting-associated protein 24, hVps24) is encoded by the CHMP3 (also known as CGI149, NEDF, VPS24, CGI-149) gene (Gene ID: 51652) in human. CHMP3 is a component of the endosomal sorting required for transport complex III (ESCRT-III), which is involved in multi-vesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. It is widely expressed and its expression is reported in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. The acidic C-terminus and the basic N-terminus of CHMP3 are thought to render the protein in a closed, soluble and inactive conformation through an auto-inhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. CHMP3 is shown to heteromerize with CHMP2A to form helical tubular structures that expose membrane-interacting sites on the outside. It can also interact with CHMP4A, however, the interaction requires the release of CHMP4A auto-inhibition.

特異性

This rabbit polyclonal antibody detects Charged multivesicular body protein 3 in human cells.

免疫原

A full-length human recombinant charged multivesicular body protein 3 (CHMP3).

Epitope: unknown

アプリケーション

Research Category
細胞シグナル伝達

Anti-CHMP3, Cat. No. ABS1668, is a rabbit polyclonal antibody that detects Charged multivesicular body protein 3 and has been tested for use in Immunocytochemistry and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected human recombinant charged multivesicular body protein 3 (CHMP3).

Western Blotting Analysis: A representative lot detected CHMP3 in Western Blotting applications (Morita, E., et. al. (2010). Proc Natl Acad Sci USA. 107(29):12889-94).

品質

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected CHMP3 in HeLa cells.

ターゲットの説明

~32 kDa observed; 25.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Unpurified

Rabbit polyclonal antiserum with 0.05% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable