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由来生物
rabbit
抗体製品の状態
unpurified
抗体製品タイプ
primary antibodies
クローン
polyclonal
交差性
mouse, human
包装
antibody small pack of 25 μL
テクニック
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
ターゲットの翻訳後修飾
unmodified
遺伝子情報
mouse ... Gstk1(76263)
詳細
Glutathione S-transferase kappa 1 (UniProt: Q9DCM2; also known as EC:2.5.1.18, GST 13-13, GST class-kappa, GSTK1-1, mGSTK1, Glutathione S-transferase subunit 13, DsbA-L) is encoded by the Gstk1 gene (Gene ID: 76263) in murine species. DsbA-L is a disulfide bond A oxidoreductase like homodimeric mitochondrial protein that is predominantly expressed in heart, kidney, liver and skeletal muscle. It is also highly expressed in adipose tissue and its expression level is negatively correlated with obesity in mice and humans. Overexpression of DsbA-L is shown to promote adiponectin multimerization. Suppression of DsbA-L expression can selectively reduce adiponectin levels and secretion in 3T3-L1 adipocytes. Expression of DsbA-L in 3T3-L1 adipocytes is shown to be stimulated by the insulin sensitizer rosiglitazone and inhibited by TNFalpha. Adiponectin expression is reduced in both human obesity and mouse models of genetic obesity. DsbA-L is also shown to co-localize with the endoplasmic reticulum (ER) marker protein disulfide isomerase and it interacts with the ER chaperone protein Ero1-L in 3T3-L1 adipocytes. Deletion of the first 6 amino acid residues at the N terminus greatly impaired DsbA-L localization in the ER. (Ref.: Liu, M et al. (2015). J Biol Chem. 290(16):10143-8).
特異性
This rabbit polyclonal antibody detects DsbA-L (Glutathione S-transferase kappa 1) in human and mouse cells.
免疫原
Epitope: unknown
Myc-tagged full length recombinant protein from mouse.
アプリケーション
Research Category
細胞シグナル伝達
細胞シグナル伝達
Anti-DsbA-L, Cat. No. ABS1644, is a rabbit polyclonal antibody that detects Glutathione S-transferase kappa 1 (DsbA-L) and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected DsbA-L in A431, HUVEC, NIH/3T3 cells.
Western Blotting Analysis: A representative lot detected DsbA-L in mouse brain, mouse kidney, and mouse liver (Liu, M., et. al. (2008). Proc Natl Acad Sci USA. 105(47):18302-7; Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).
Immunoprecipitation Analysis: A representative lot detected DsbA-L in 3T3-L1 adipocytes (Liu, M., et. al. (2008). Proc Natl Acad Sci USA. 105(47):18302-7; Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).
Immunocytochemistry Analysis: A representative lot detected DsbA-L in adipocytes on differentiation day 7 were used for the following studies. confocal immunofluorescence study of DsbA-L co-localization with the mitochondrial marker (MitoTracker) and the ER marker (PDI) in 3T3-L1 adipocytes (Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).
Western Blotting Analysis: A representative lot detected DsbA-L in mouse brain, mouse kidney, and mouse liver (Liu, M., et. al. (2008). Proc Natl Acad Sci USA. 105(47):18302-7; Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).
Immunoprecipitation Analysis: A representative lot detected DsbA-L in 3T3-L1 adipocytes (Liu, M., et. al. (2008). Proc Natl Acad Sci USA. 105(47):18302-7; Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).
Immunocytochemistry Analysis: A representative lot detected DsbA-L in adipocytes on differentiation day 7 were used for the following studies. confocal immunofluorescence study of DsbA-L co-localization with the mitochondrial marker (MitoTracker) and the ER marker (PDI) in 3T3-L1 adipocytes (Lui, M., et. al. (2015). J Biol Chem. 290(16):10143-8).
品質
Evaluated by Western Blotting in mouse kidney tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DsbA-L in 10 µg of mouse kidney tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DsbA-L in 10 µg of mouse kidney tissue lysate.
ターゲットの説明
~28 kDa observed; 25.70 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
物理的形状
Unpurified
Format: Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide with 20% glycerol.
Rabbit polyclonal antiserum with 0.05% sodium azide with 20% glycerol.
保管および安定性
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABS1644-25UL:
ABS1644:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Juli Bai et al.
Communications biology, 3(1), 257-257 (2020-05-24)
Obesity is a global epidemic that is caused by excessive energy intake or inefficient energy expenditure. Brown or beige fat dissipates energy as heat through non-shivering thermogenesis by their high density of mitochondria. However, how the mitochondrial stress-induced signal is
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