ABF1070

Anti-DRBP76/ILF3 Antibody

serum, from rabbit

• 別名: Interleukin enhancer-binding factor 3, Double-stranded RNA-binding protein 76, DRBP76, M-phase phosphoprotein 4, MPP4, Nuclear factor associated with dsRNA, NFAR, Nuclear factor of activated T-cells 90 kDa, NF-AT-90, Translational control protein 80, TCP
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 交差性: human
• テクニック: western blot: suitable
• NCBIアクセッション番号: NP_036350
• UniProtアクセッション番号: Q12906
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ILF3(3609)

詳細

詳細

Interleukin enhancer-binding factor 3 (UniProt Q12906; also known as Double-stranded RNA-binding protein 76, DRBP76, dsRNA binding protein NFAR-2/MPP4, Interleukin enhancer binding factor 3 90kD, M-phase phosphoprotein 4, MPP4, NF-AT-90, NFAR, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, TCP80, Translational control protein 80) is encoded by the ILF3 (also known as DRBF, NF90, MPHOSPH4) gene (Gene ID 3609) in human. DRBP76 is a dsRNA binding protein (DRBP) family member capable of binding both dsRNAs and highly structured single-stranded RNA molecules through its C-terminal region. DRBP76 influences the nucleus-cytoplasm export, stability, and translation of target mRNAs via direct interaction with their 3′ UTRs. DRBP76 is also reported to play both positive and negative regulatory roles in viral replications. DRBP76 is shown to interact with the 3′-terminal stem loop (SL) structure of the single-stranded RNA genome of the dengue virus and DRBP76 knockdown decreases production of functional dengue virus in cultured cells. On the other hand, DRBP76 can interact with full-length Zaire Ebola virus (EBOV) protein VP35 in the absence of exogenous dsRNA. Viral VP35 expression redistributes DRBP76 from the nucleus to the cytoplasm in the host cells and over-expression of DRBP76 impairs the function of the EBOV transcription/replication complex.

特異性

Expected to react with all 7 spliced isoforms.

免疫原

His-tagged recombinant protein corresponding to human DRBP76/ILF3.

アプリケーション

This Anti-DRBP76/ILF3 Antibody is validated for use in Western Blotting for the detection of DRBP76/ILF3.

Western Blotting Analysis: A representative lot detected DRBP76/ILF3 in the nuclear fractions prepared from the squamous cell carcinoma of the head and neck (SCCHN) cell lines PCI-15A and PCI-15B (Umemura, N., et al, (2012). Cancer Res. 72(1):45-55).
Western Blotting Analysis: A representative lot detected DRBP76/ILF3 in the nuclear fractions prepared from Wt11 (HEK293 expressing Flag-tagged wild-type TLR3) cells (Zhu, J., et al, (2010). J Immunol. 184(10):5768-5776; Sarkar, S.N., et al, (2004). Nat Struct Mol Biol. 11(11):1060-1067).

品質

Evaluated by Western Blotting in MDA-MB-435 cell lysate.

Western Blotting Analysis: A 1:500 of this antibody detected DRBP76/ILF3 in 10 µg of MDA-MB-435 cell lysate.

ターゲットの説明

~100 kDa A lower size band ~75 kDa may be seen in some lysates, most likely representing a smaller size variant or a non-specific band. Uncharacterized band(s) may appear in some lysates.

その他情報

Concentration: Please refer to lot specific datasheet.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable