MABC609

Anti-Thymidylate Kinase/TMPK Antibody, clone 1A5.1

clone 1A5.1, from mouse

• 別名: Thymidylate kinase, Thymidylate Kinase/TMPK, dTMP kinase
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 1A5.1, monoclonal
• 化学種の反応性: human
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_001158503
• UniProtアクセッション番号: P23919
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TK1(7083)

詳細

詳細

Thymidylate kinase (EC 2.7.4.9; UniProt P23919; also known as dTMP kinase) is encoded by the DTYMK (also known as CDC8, PP3731, TMPK, TYMK) gene (Gene ID 1841) in human. Thymidylate kinase (TMPK) is an ubiquitously expressed enzyme of the dTTP synthesis pathway involved in DNA synthesis. TMPK catalyzes the phosphorylation of thymidine 5′-monophosphate (dTMP) in the presence of ATP and Mg(2+) to form thymidine 5′-diphosphate (dTDP), which is then further phosphorylated by nucleoside-diphosphate kinase (NDK) to thymidine 5′-triphosphate (dTTP). Aside from its normal physiological role, TMPK activity is essential for the activation of nucleoside analogs-based prodrugs, such as the clinically used anti-HIV prodrugs AZT and d4T, which need to be converted to the triphosphate forms for their pharmacological activities. Inhibition of TMPK activity is reported to augment the potency of anticancer agent doxorubicin against human colon cancer cells regardless of their p53 status. In addition, lung cancers with LKB1-mutations are reported to show deficits in nucleotide metabolism and exhibit hypersensitivity to DTYMK inhibition.

免疫原

GST-tagged recombinant protein corresponding to human Thymidylate Kinase/TMPK.

アプリケーション

Research Category
アポトーシス及び癌

Research Sub Category
アポトーシス-追加

This Anti-TMPK Antibody, clone 1A5.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Thymidylate Kinase .

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Thymidylate Kinase/TMPK in 10 µg of MCF-7 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Thymidylate Kinase/TMPK in human breast cancer and human ovarian cancer tissue.

品質

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Thymidylate Kinase/TMPK in 10 µg of Jurkat cell lysate.

ターゲットの説明

~24 kDa observed

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable