ABE2909

Anti-phospho Histone H4 (Tyr51)

from rabbit

• 別名: H4, H4Y51, H4pY51
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: human
• 包装: antibody small pack of 25 μL
• テクニック: immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_778224 (Multiple NP# numbers)
• UniProtアクセッション番号: P62805
• 輸送温度: ambient
• ターゲットの翻訳後修飾: phosphorylation (pTyr51)
• 遺伝子情報: human ... HIST2H4B(554313)

詳細

詳細

Histone H4 (UniProt: P62805 ) is encoded by the HIST14A (also known as H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4) gene (Gene ID: 121504, 554313, 8294, 8359, 8360, 8361, 8362, 8363, 8364, 8365, 8366, 8367, 8368, 8370) in human. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H4 is one of the main histone proteins that contribute to the chromatin structure in both prokaryotic and eukaryotic cells. Histone H4 can undergo several different types of epigenetic modifications that influence cellular processes, including methylation, acetylation, and phosphorylation. Acetylation at Lys6, Lys9, Lys13 and Lys17 is shown to occur in coding regions of the genome but not in heterochromatin. Monomethylation and asymmetric dimethylation at Arg4 is reported to favor acetylation at Lys9 and Lys13. Histone H4 can be phosphorylated by Tie2 on Tyr51, which is shown to preferentially occur in the presence of core histones. The phosphorylated H4 (H4Y51) is recognized by the proto-oncogene ABL1. Histone H4 is also phosphorylated on Ser48 by PAK2, which is shown to increase the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Chromosomal aberrations involving histone H4 causes of B-cell non-Hodgkin lymphomas. (Ref.: Hossain, MB., et al. (2016). Sci. Adv. 2; e1501290).

特異性

This rabbit polyclonal antibody detects Histone H4 phosphorylated on Tyrosine 51. It targets an epitope within 11 amino acids surrounding phosphotyrosine 51.

免疫原

Epitope: unknown

Two linear peptides (phosphorylated on Tyr 51 and non-phosphorylated) corresponding to 11 amino acids from the internal region of human Histone H4.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Anti-phospho Histone H4 (Tyr51) Antibody, Cat. No. ABE2909, is a highly specific rabbit polyclonal antibody that targets Histone H4 phosphorylated on Tyr51 and has been tested for use in in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Western Blotting Analysis: A representative lot detected Tyr51 phosphorylation of Histone H4 in the presence of active recombinant Tie2, following analysis by Western blotting. (Hossain, MB., et al. (2016). Sci. Adv. 2; e1501290).

Immunofluorescence Analysis: A representative lot detected phospho Histone H4 (Tyr51) in HEK293 Tie2 cells treated with Angiopoietin 1 (ANG1) and ionizing radiation (Hossain, MB., et al. (2016). Sci. Adv. 2; e1501290).

品質

Evaluated by Immunocytochemistry in HEK293 Tie2-myc cells treated with Angiopoietin 1 (ANG1).

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected phospho Histone H4 (Tyr51) in HEK293 Tie2-myc cells treated with Angiopoietin 1 (ANG1).

ターゲットの説明

11.38 kDa calculated.

物理的形状

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody containing PBS without preservatives and 40% glycerol.

保管および安定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): does not flash
• 引火点(℃): does not flash