おすすめの製品
由来生物
rabbit
品質水準
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
mouse
化学種の反応性（ホモロジーによる予測）
human (based on 100% sequence homology), rat (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology)
テクニック
ChIP: suitable (ChIP-seq)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... TET3(200424)
詳細
MABE1144/1132/1133 Methylcytosine dioxygenase TET3 (EC 1.14.11.n2; UniProt O43151) is encoded by the TET3 (also known as KIAA0401) gene (Gene ID 200424) in human. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported. Three TET3 spliced isoforms/variants have been reported, the long or full-length isoform Tet3FL and the short isoform Tet3s are upregulated during embryonic stem cell (ESC) differentiation toward the neuronal lineage, while Tet3o is oocyte-specific and not detected in ESCs or in any other cell types or adult mouse tissues tested, indicating a role of Tet3o in paternal genome oxidation in mouse zygotes. Tet3FL and Tet3o contain the entire Tet3s sequence with additional (but different) N-terminal sequence. Only Tet3FL contains an N-terminal CXXC domain that binds to unmethylated CpGs and exhibits highest affinity toward 5caC. Tet3FL is substantially less active than Tet3o and Tet3s, suggesting that the CXXC domain restricts Tet3FL 5mC oxidation capacity.
特異性
Immunogen sequence is present in TET3 isoforms Tet3FL, Tet3s, and Tet3o spliced isoforms reported by Jin, S.G., et al. (2016). Cell Rep. 14(3):493-505), as well as all additional human and mouse TET3 spliced isoforms reported by UniProt (O43151, Q8BG87). Note that UniProt isoform 1 (O43151-1/human and Q8BG87-1/mouse) corresponds to isoform Tet3s, not Tet3FL (NM_001287491.1/human and JX946278.1/mouse).
免疫原
KLH-conjugated linear peptide corrsponding to an internal sequence within the N-terminal half of human TET3.
アプリケーション
Anti-TET3, Cat. No. ABE290, is a highly specific rabbit polyclonal antibody that targets methylcytosine dioxygenase TET3 and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated the exogenously expressed mouse TET3-GFP fusion protein from 500 µg lysate of transfected mouse embryonic stem cells (mESCs).
Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected TET3 chromosome enrichment sites by ChIP-seq analysis (10 µg per ChIP) using chromatin preparation from mouse embryonic stem cells (mESCs) transfected to overexpress mouse TET3 (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot immunostained 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized mouse embryonic stem cells (mESCs) transfected to overexpress mouse TET3 oocyte isoform Tet3o (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected the immunoreactivity of TET3 oocyte isoform Tet3o in 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized mouse zygotes (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunoprecipitation Analysis: 10 µg from a representative lot co-immunoprecipitated endogenous OGT with the exogenously overexpressed mouse TET3 from transfected mouse embryonic stem cells (mESCs) by RIME (Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins) (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected TET3 chromosome enrichment sites by ChIP-seq analysis (10 µg per ChIP) using chromatin preparation from mouse embryonic stem cells (mESCs) transfected to overexpress mouse TET3 (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot immunostained 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized mouse embryonic stem cells (mESCs) transfected to overexpress mouse TET3 oocyte isoform Tet3o (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected the immunoreactivity of TET3 oocyte isoform Tet3o in 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized mouse zygotes (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunoprecipitation Analysis: 10 µg from a representative lot co-immunoprecipitated endogenous OGT with the exogenously overexpressed mouse TET3 from transfected mouse embryonic stem cells (mESCs) by RIME (Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins) (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
品質
Evaluated by Western Blotting of mouse TET3 GFP fusion.
Western Blotting Analysis: 2 µg/mL of this antibody detected the exogenously expressed mouse TET3 GFP fusion protein in 10 µg of lysate from transfected mouse embryonic stem cells (mESCs).
Western Blotting Analysis: 2 µg/mL of this antibody detected the exogenously expressed mouse TET3 GFP fusion protein in 10 µg of lysate from transfected mouse embryonic stem cells (mESCs).
ターゲットの説明
193.7/179.4 kDa (human isoform Tet3FL/Tet3s), 194.8/180.4/185.1 kDa (mouse isoform Tet3FL/Tet3s/Tet3o), 166.7/77.47 kDa (UniProt human isoform 2/3), 76.26 kDa (UniProt mouse isoform 2) calculated. Note that UniProt isoform 1 (O43151-1/human and Q8BG87-1/mouse) corresponds to isoform Tet3s, not Tet3FL (NM_001287491.1/human and JX946278.1/mouse).
保管および安定性
Stable for 1 year at 2-8°C from date of receipt..
その他情報
Concentration: Please refer to lot specific datasheet.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABE290:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Aixia Liu et al.
iScience, 24(9), 103065-103065 (2021-09-28)
A conceptual framework for understanding abnormal endometrial decidualization, with considerable significance for the diagnosis and treatment of abnormal decidualization-related changes in non-receptive endometrium in implantation failure during early pregnancy is very important. Here, we found the expression levels of miR-29a
Tiefeng Cao et al.
Oncogene, 38(27), 5356-5366 (2019-05-16)
Uterine leiomyomas or fibroids (UFs) are benign tumors characterized by hyperplastic smooth muscle cells and excessive deposition of extracellular matrix (ECM). Afflicting ~80% of women, and symptomatic in 25%, UFs bring tremendous suffering and are an economic burden worldwide; they
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）