ABE2867M

Anti-NR5A2 (LRH1)

from rabbit

• 別名: Nuclear receptor subfamily 5 group A member 2, Alpha-1-fetoprotein transcription factor, B1-binding factor, hB1F, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, LRH-1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: unpurified
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 交差性: human, mouse
• 包装: antibody small pack of 25 μL
• テクニック: ChIP: suitable (ChIP-seq); immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_995582
• UniProtアクセッション番号: O00482
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... NR5A2(2494)

詳細

詳細

Nuclear receptor subfamily 5 group A member 2 (UniProt: O00482; also known as Alpha-1-fetoprotein transcription factor, B1-binding factor, hB1F, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, LRH-1) is encoded by the NR5A2 (also known as B1F, CPF, FTF) gene (Gene ID: 2494) in human. It is a member of the nuclear receptor superfamily that binds to the sequence element 5′-AACGACCGACCTTGAG-3′ of the enhancer II of hepatitis B virus genes. It contains a DNA binding region (aa 83-104) and two NR C4-type zinc finger domains (aa 86-106 and 122-146). It is abundantly expressed in pancreas, but at reduced levels in liver, and at very low levels in heart and lung. It s expression has been demonstrated in the Hep-G2 cell line. Four different isoforms of LRH-1 have been described that are generated by alternate splicing. LRH-1 is reported to be involved in the regulation of expression of transcription factors, such as Oct4, HNF1, 3, and 4, which are known to participate in embryonic development. Its presence in high levels in beta-III Tubulin+ and NeuN+ neurons of the mantle zone (MZ) in early neural tube of mice (E10.5 and E12.5) indicate its involvement in neural differentiation. (Ref.: Stergiopoulos, A., and Politis, PK (2016). Nat. Commun. DOI: 10.1038/ncomms12230; Venteclef, N et al. (2008). J. Biol. Chem. 283(7); 3694 3701).

特異性

This rabbit polyclonal antibody detects Nuclear receptor subfamily 5 group A member 2 (NR5A2) in human and murine cells.

免疫原

Epitope: unknown

His-tagged full length recombinant human Nuclear receptor subfamily 5 group A member 2, isoform 2.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Anti-NR5A2 (LRH1), Cat. No. ABE2867, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 5 group A member 2 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunofluorescence, Immunoprecipitation, and Western Blotting.

Chromatin Immunoprecipitation Analysis: A representative lot detected NR5A2 (LRH1) in Chromatin Immunoprecipitation applications (Kaltezioti, V., et. al. (2010). PLoS Biol. 8(12):e1000565; Venteclef, N., et. al. (2010). Genes Dev. 24(4):381-95; Boulias, K., et. al. (2005). EMBO J. 24(14):2624-33; Chalkiadaki, A., et. al. (2005). Mol Cell Biol. 25(12):5095-105).

Western Blotting Analysis: A representative lot detected NR5A2 (LRH1) in Western Blotting applications (Chalkiadaki, A., et. al. (2005). Mol Cell Biol. 25(12):5095-105; Stergiopoulos, A., et. al. (2016). Nat Commun. 7:12230).

Immunofluorescence Analysis: A representative lot detected NR5A2 (LRH1) in Immunofluorescence YES titration paraffin sections from E10.5 (a), E12.5 (b), E14.5 (c) or E16.5 (d) mouse spinal cord (Stergiopoulos, A., et. al. (2016). Nat Commun. 7:12230).

品質

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NR5A2 (LRH1) in 10 µg of HEK293 cell lysate.

ターゲットの説明

~68 kDa observed; 61.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Unpurified

Format: Unpurified

Rabbit polyclonal antiserum with 0.05% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable