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由来生物
rabbit
品質水準
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
交差性
human, mouse, rat
テクニック
immunocytochemistry: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
遺伝子情報
mouse ... Pdcd6Ip(18571)
詳細
Programmed cell death 6-interacting protein (UniProt: Q9WU78; also known as ALG-2-interacting protein 1, ALG-2-interacting protein X, E2F1-inducible protein, Eig2) is encoded by the Pdcd6ip (also known as Aip1, Alix) gene (Gene ID: 18571) in murine species. AIP1 is a class E VPS cytoplasmic, ubiquitously expressed protein that is involved in concentration and sorting of cargo proteins of the multi-vesicular body (MVB) for incorporation into intra-lumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. AIP1 interacts with ALG-2 (apoptosis-linked gene-2) to regulate cell death. This interaction is strictly dependent on calcium. AIP1 is considered to be essential for the maintenance of fibroblast morphology and has been shown to play a role in the regulation of both apoptosis and cell proliferation. AIP1 is also known to regulate exosome biogenesis in concert with SDC1/4 and SDCBP. AIP1 can undergo phosphorylated on tyrosine residues by activated platelet-derived growth factor receptor-beta (PDGFRB).
特異性
This polyclonal antibody specifically detects AIP1 in Jurkat cells. It is likely to react with isoforms 1, 2, and 3.
免疫原
GST-tagged recombinant fragment corresponding to 479 amino acids from the C-terminal half of mouse AIP1/Alix.
アプリケーション
Research Category
アポトーシス及び癌
アポトーシス及び癌
Research Sub Category
アポトーシス-追加
アポトーシス-追加
Detect Programmed cell death 6-interacting protein using this rabbit polyclonal Anti-AIP1/Alix, Cat. No. ABC40 that has been tested in Immunocytochemistry and Western blotting.
Immunocytochemistry Analysis: 2.5 µg/mL from a representative lot detected AIP1/Alix in HeLa and NIH3T3 cells.
品質
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected AIP1/Alix in 10 µg of Jurkat cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected AIP1/Alix in 10 µg of Jurkat cell lysate.
ターゲットの説明
~95 kDa observed; 96.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
物理的形状
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 20 mM Tris-HCl, pH 7.2, 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
アナリシスノート
Control
HeLa cell lysate
HeLa cell lysate
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABC40:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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