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由来生物
rabbit
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
polyclonal
交差性
bovine
メーカー/製品名
Chemicon®
テクニック
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
radioimmunoassay: suitable
適合性
not suitable for Western blot
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
遺伝子情報
human ... COL1A1(1277)
特異性
Reacts with bovine collagen type I collagen.
Antibody shows less than 0.1% cross reactivity in RIA at 1:500 dilution with bovine collagen types II, IX & XI, or fibronectin; other collagens and ECM proteins untested.
Reactivity with human collagen type I <2.0% with RIA, 1:500; reactivity with Chicken collagen type I, less than 0.1% by RIA at 1:500 dilution.
Antibody shows less than 0.1% cross reactivity in RIA at 1:500 dilution with bovine collagen types II, IX & XI, or fibronectin; other collagens and ECM proteins untested.
Reactivity with human collagen type I <2.0% with RIA, 1:500; reactivity with Chicken collagen type I, less than 0.1% by RIA at 1:500 dilution.
免疫原
purified bovine type I collagen extracted from purified bovine skin.
アプリケーション
Research Category
細胞骨格
細胞骨格
Research Sub Category
ECMタンパク質
ECMタンパク質
Anti-Collagen Type I Antibody detects level of Collagen Type I & has been published & validated for use in ELISA, RIA, IH, IH(P).
Immunohistochemistry: 1:40 dilution for immunofluorescent staining of frozen bovine liver and skin tissues.
Immunohistochemistry on 2% formalin or PFA fixed, paraffin embedded tissues requires HEIR antigen retrieval and enhanced enzymatic detection; methyl-carnoy, acetone, or acid-aldehyde fixation can enhance reactivity for paraffin sections.
Radioimmunoassay
ELISA: 1:200 direct
Not recommended for Western blots.
Immunohistochemistry on 2% formalin or PFA fixed, paraffin embedded tissues requires HEIR antigen retrieval and enhanced enzymatic detection; methyl-carnoy, acetone, or acid-aldehyde fixation can enhance reactivity for paraffin sections.
Radioimmunoassay
ELISA: 1:200 direct
Not recommended for Western blots.
関連事項
Replaces: AB749
物理的形状
Format: Purified
Protein G purified IgG fraction in liquid at 1 mg/mL in 0.01M phosphate, 0.09M NaCl, pH 7.2.No preservatives.
保管および安定性
Maintain frozen at -20°C for up to 12 months. Avoid repeated freeze/thaw cycles
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB749P:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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PeerJ null
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