AB5613P

Anti-Brain Derived Neurotrophic Factor Antibody, pro

Chemicon^®, from rabbit

• 別名: Prepro-Abrineurin, Pro-Abrineurin, Prepro-BDNF, Pro-BDNF, Prepro-Brain-derived neurotrophic factor, Pro-Brain-derived neurotrophic factor
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse, rat, human
• メーカー／製品名: Chemicon^®
• テクニック: immunohistochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NM_001143809; NM_001143810; NM_001709; NM_170731; NM_170734
• UniProtアクセッション番号: P23560
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... BDNF(627)

詳細

詳細

Brain-derived neurotrophic factor (UniProt P23560; also known as Abrineurin, BDNF, Neurotrophin) is encoded by the BDNF (also known as BULN2) gene (Gene ID 627) in human. BDNF is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT3, and NT4/5. BDNF mediates its neurotrophic properties by signaling through the high affinity cell surface receptor gp145/trkB. BDNF is initially produced with a signal peptide (a.a. 1-18) and a propeptide (a.a. 19-128) sequence, the removal of which yields the mature BDNF (a.a. 129-247).

特異性

This polyclonal antibody detects prepro- and pro-BDNF, as well as prepro-/pro-BDNF-derived N-terminal fragments that contain the propeptide sequence (a.a. 19-128), but not mature BDNF or C-terminal fragments without the propeptide sequence. No cross-reactivity to other proneurotrophins.

免疫原

Epitope: Propeptide.

KLH-conjugated linear peptide corresponding to a region within human BDNF propeptide sequence.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
神経化学・ニューロトロフィン

神経炎症及び疼痛

Anti-Brain Derived Neurotrophic Factor Antibody, pro is an antibody proBDNF for use in Immunohistochemistry and Western Blotting.

Western blot: 1:2,000

Immunohistochemistry: 1:2,000

Optimal working dilutions must be determined by the end user.

ターゲットの説明

~35 kDa (glycosylated prepro-/pro-BDNF), ~50-65 kDa (glycosylated prepro-/pro-BDNF dimer), and ~26-28 kDa (non-glycosylated prepro/pro-BDNF or glycosylated prepro-/pro-BDNF N-terminal fragments) observed. Target bands appear larger than the calculated molecular weights of 27.82/28.87/29.82/37.12/31.12 kDa (human prepro-BDNF isoform 1/2/3/4/5) and 25.77/26.82/27.77/35.07/29.06 kDa (human pro-BDNF isoform 1/2/3/4/5) due to glycosylation and/or dimerization. Uncharacterized band(s) may appear in some lysates.

物理的形状

Lyophilized from 10 mM PBS. Reconstitute with 20 μL of sterile distilled water.

保管および安定性

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C undiluted for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added (1:1) for additional stability.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable