AB3565

Anti-PARP (214/215) cleavage site Antibody

Chemicon^®, from rabbit

• 別名: Poly [ADP-ribose] polymerase 1, PARP-1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, ARTD1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse, bovine, rat, human
• メーカー／製品名: Chemicon^®
• テクニック: immunocytochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NM_001618.3
• UniProtアクセッション番号: P09874
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: bovine ... Parp1(286764) ; human ... PARP1(142) ; mouse ... Parp1(11545) ; rat ... Parp1(25591)

詳細

詳細

Poly (ADP-Ribose) Polymerase (PARP) is a 116 kDa nuclear protein which is strongly activated by binding to DNA strand breaks. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.

特異性

Reactivity with other species has not been confirmed. This sequence is 86% homologous in mouse, rat, and hamster.

The antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.

免疫原

Synthetic peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP.

アプリケーション

Research Category
アポトーシス及び癌

代謝

Research Sub Category
アポトーシス-追加

酵素及び生化学

Anti-PARP (214/215) cleavage site Antibody detects level of PARP (214/215) cleavage site & has been published & validated for use in WB & IC.

For Western blot applications, we recommend using the antibody at a 1:1,000 starting dilution.

品質

Purified from rabbit serum by affinity chromatography using a peptide corresponding to the PARP cleavage site.

関連事項

Replaces: 04-575; 04-576

物理的形状

ImmunoAffinity Purified

Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide

保管および安定性

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.

アナリシスノート

Control
Jurkat and HeLa cells treated with staurosporine or etoposide (25 µM for 3 hours).

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable