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由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
human, mouse, chicken, hamster, rat, pig
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
chicken ... Itga4(424121)
human ... ITGA4(3676)
mouse ... Itga4(16401)
pig ... Itga4(100521477)
rat ... Itga4(311144)
特異性
Integrin alpha 4. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine-labeled lymphoid cells. No cross-reactivity to alpha 1, alpha 2, alpha 3, alpha 5, alpha 6 or alpha V. The antibody recognizes an epitope on the cytoplasmic domain of the protein, and it is thus intracellular.
免疫原
Epitope: cytoplasmic domain, C-terminus
Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha 4 integrin subunit.
アプリケーション
Research Category
細胞骨格
細胞骨格
Research Sub Category
インテグリン
インテグリン
Anti-Integrin α4 Antibody, cytoplasmic on C-terminus is an antibody against Integrin α4 for use in ELISA, IP, RIA, WB, IH.
ELISA/RIA: 1:1000.
Western blot: 1:1000, non-reduced conditions; blocking should be with 1-3% BSA. Recognizes a 140 kDa human alpha 4 band under non-reducing conditions.
Immunohistochemistry: 1:500
Immunoprecipitation: 5 μL of antibody is sufficient to precipitate alpha4 from 5x106 cells.
Optimal working dilutions must be determined by end user.
Western blot: 1:1000, non-reduced conditions; blocking should be with 1-3% BSA. Recognizes a 140 kDa human alpha 4 band under non-reducing conditions.
Immunohistochemistry: 1:500
Immunoprecipitation: 5 μL of antibody is sufficient to precipitate alpha4 from 5x106 cells.
Optimal working dilutions must be determined by end user.
関連事項
Replaces: 04-1131
物理的形状
Liquid rabbit antiserum with 0.05% sodium azide.
保管および安定性
Maintain at -20ºC in undiluted aliquots for up to 12 months upon date of receipt. Avoid repeated freeze/thaw cycles.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Regulation of human osteoblast integrin expression by orthopedic implant materials.
R K Sinha, R S Tuan, R K Sinha, R S Tuan, R K Sinha, R S Tuan
Bone null
J Linfor et al.
Journal of reproduction and fertility, 120(1), 65-72 (2000-09-28)
Integrin molecules are cell adhesion molecules that are thought to be involved in sperm-oocyte interaction in rodents and humans. The objective of this study was to evaluate whether integrin molecules were present on the surface of pig oocytes, consistent with
Regulation of integrin function by CD47 ligands. Differential effects on alpha vbeta 3 and alpha 4beta1 integrin-mediated adhesion.
Barazi, HO; Li, Z; Cashel, JA; Krutzsch, HC; Annis, DS; Mosher, DF; Roberts, DD
The Journal of Biological Chemistry null
Expression of alphav, alpha4, alpha5 and beta3 integrin subunits, fibronectin and vitronectin in goat peri-implantation
Garcia, P. et al.
Animal Reproduction Science, 80(1-2), 91-100 (2004)
Integrins mediate adherence and migration of T lymphocytes on human peritoneal mesothelial cells.
Wang, HH; Lee, TY; Lin, CY
Kidney International null
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