8.52095

Fmoc-Lys(Mca)-OH

≥97.0% (HPLC), for peptide synthesis, Novabiochem^®

• 別名: Fmoc-Lys(Mca)-OH, N-α-Fmoc-N-ε-7-methoxycoumarin-4-acetyl-L-lysine
• 実験式（ヒル表記法）: C₃₃H₃₂N₂O₈
• CAS番号: 386213-32-7
• 分子量: 584.62
• UNSPSCコード: 12352209
• NACRES: NA.22

特性

• product name: Fmoc-Lys(Mca)-OH, Novabiochem^®
• 品質水準: 200
• 製品種目: Novabiochem^®
• アッセイ: ≥97% (TLC); ≥97.0% (HPLC)
• 形状: powder
• 反応適合性: reaction type: Fmoc solid-phase peptide synthesis
• メーカー／製品名: Novabiochem^®
• アプリケーション: peptide synthesis
• 官能基: amine
• 保管温度: −20°C
• InChI: 1S/C33H32N2O8/c1-41-21-13-14-22-20(17-31(37)43-29(22)18-21)16-30(36)34-15-7-6-12-28(32(38)39)35-33(40)42-19-27-25-10-4-2-8-23(25)24-9-3-5-11-26(24)27/h2-5,8-11,13-14,17-18,27-28H,6-7,12,15-16,19H2,1H3,(H,34,36)(H,35,40)(H,38,39)/t28-/m0/s1
• InChI Key: BWJJONDPLYEAMC-NDEPHWFRSA-N

詳細

詳細

A modified lysine derivative for the preparation of fluorogenically-labeled peptides by Fmoc SPPS ^[1]. The Mca group fluoresces at 405 nm when stimulated at 340 nm, and is most commonly used in conjunction with Dabcyl and 2,4-dinitrophenyl quenching groups. Recently, Fmoc-Lys(Mca)-OH has been used to prepare lanthanide-based luminescent proteins by native chemical ligation ^[2]. Lys(Mca) was found to be stable to HF cleavage conditions used to synthesize the thioester fragments needed for ligation.

Associated Protocols and Technical Articles
Cleavage and Deprotection Protocols for Fmoc SPPS
Chromogenic Labeling of Synthetic Peptides

Literature references

^[1] J. L. Lauer-Fields, et al. (2001) Biochemistry, 40, 5795.
^[2] C. F. W. Becker, et al. (2004) Bioconjugate Chem., 15, 1118.

関連事項

Replaces: 04-12-1233

アナリシスノート

Color (visual): white to slight yellow to beige
Appearance of substance (visual): powder
Identity (IR): passes test
Enantiomeric purity: ≥ 99.0 % (a/a)
Purity (TLC(011A)): ≥ 97 %
Assay (HPLC, area%): ≥ 97.0 % (a/a)
Water (K. F.): ≤ 2.0 %

To see the solvent systems used for TLC of Novabiochem^® products please click here.

法的情報

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable