8.51025

5-Carboxyfluorescein

Novabiochem^®

• 別名: 5-Carboxyfluorescein, 5-FAM
• 実験式（ヒル表記法）: C₂₁H₁₂O₇
• CAS番号: 76823-03-5
• 分子量: 376.32
• MDL番号: MFCD00036874
• UNSPSCコード: 12352005
• NACRES: NA.22

特性

• 品質水準: 200
• 製品種目: Novabiochem^®
• アッセイ: ≥98% (TLC); ≥99.0% (HPLC)
• フォーム: powder
• 反応適合性: reagent type: fluorescent-labelling reagent
• メーカー／製品名: Novabiochem^®
• アプリケーション: peptide synthesis
• 保管温度: 2-8°C
• SMILES記法: O1C3(c4c(cc(cc4)O)Oc5c3ccc(c5)O)c2c(cc(cc2)C(=O)O)C1=O
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
• InChI Key: NJYVEMPWNAYQQN-UHFFFAOYSA-N

詳細

詳細

This high purity fluorescent dye (λ_ex494 nm, λ_em 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or be protected by tritylation prior to acylation or hydrazine treatment ^[1].

Associated Protocols and Technical Articles
Chromogenic Labeling of Synthetic Peptides

Literature references

^[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.

アプリケーション

5-Carboxyfluorescein Novabiochem^®. CAS 76823-03-5, molar mass 376.32 g/mol.

関連事項

Replaces: 01-63-0112

アナリシスノート

Colour (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 98 %
Assay (HPLC, area%): ≥ 99.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem^® products please click here.

法的情報

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable