71206

Benzonase^® Nuclease HC

Purity > 99%, Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions

• 別名: Endonuclease from Serratia marcescens
• CAS番号: 9025-65-4
• Enzyme Commission number: 3.1.30.2 (BRENDA, IUBMB)
• MDL番号: MFCD00131010
• UNSPSCコード: 12352202
• NACRES: NA.54

特性

• product name: エンドヌクレアーゼ Serratia marcescens（セラチア菌）由来, Effective Viscosity Reduction and Removal of Nucleic Acids from protein solutions
• 由来生物: Serratia marcescens
• 品質水準: 100
• リコンビナント: expressed in E. coli
• アッセイ: >99% (SDS-PAGE)
• 形状: buffered aqueous glycerol solution
• メーカー／製品名: Novagen^®
• 保管条件: OK to freeze
• 濃度: ≥250 units/μL
• 不純物: <0.25 EU/kU Total endotoxin
• アプリケーション: research use
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

Benzonase^® Nuclease is a geneticallyengineered endonuclease from Serratiamarcescens. It degrades all forms of DNA andRNA (single stranded, double stranded, linearand circular) while having no proteolyticactivity. It is effective over a wide range ofconditions and possesses an exceptionallyhigh specific activity. The enzyme completelydigests nucleic acids to 5′-monophosphateterminated oligonucleotides 2 to 5 bases inlength (below the hybridization limit), whichis ideal for removal of nucleic acids fromrecombinant proteins, enabling compliancewith FDA guidelines for nucleic acidcontamination. The ability of Benzonase torapidly hydrolyze nucleic acids makes theenzyme an excellent choice for viscosityreduction to reduce processing time andincrease yields of protein. For example, theenzyme is compatible with BugBuster andPopCulture Protein Extraction Reagentsand can therefore be added along with thesereagents to eliminate viscosity and removenucleic acids from E. coli extracts.

The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42°C and requires1-2 mM Mg²⁺ for activation. The enzyme is also active in the presence of ionic and non-ionicdetergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by >150 mM monovalentcations, >100 mM phosphate, >100 mMammonium sulfate, or >100 mM guanidine HCl.

Benzonase Nuclease is available inultrapure (>99% by SDS-PAGE) and pure(>90%) grades at a standard concentrationof 25-29 U/µl and at a high concentration (HC)of 250 U/µl. Both preparations are free ofdetectable protease and have specific activity> 1 × 10⁶ U/mg protein. The >99% puritygrade is tested for endotoxins and contains<0.25 EU/1000 units. The product is suppliedas a 0.2 µm filtered solution in 50% glycerol.Store at -20°C.

Total endotoxin:<0.25 EU/1,000 units.Purity: >99% by SDS-PAGE.

アプリケーション

タンパク質サンプルから核酸を除去する際に使用します。

生物化学的／生理学的作用

未変性または熱変性のDNA, RNAを分解します。

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.

法的情報

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2