おすすめの製品
フォーム
liquid
メーカー/製品名
Novagen®
保管条件
OK to freeze
輸送温度
wet ice
関連するカテゴリー
詳細



Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis
In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind<TMSYMBOL></TMSYMBOL> and Ni-NTA HIS-BIND® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.
特徴および利点
- Gentle, rapid, efficient extraction of proteins from yeast cells
- Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
- Higher yield of total and enzymatically active proteins
- Fully compatible with Ni-NTA His•Bind and GST•Bind affinity purification methods
構成
•1 ml or 5 ml100X THP Solution
警告
その他情報
法的情報
シグナルワード
Warning
危険有害性情報
危険有害性の分類
STOT RE 2 Oral
ターゲットの組織
Kidney
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(℃)
Not applicable
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast
YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast
関連コンテンツ
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
界面活性剤可溶化、凍結融解溶解、浸透圧ショック、超音波処理、酵素細胞溶解、および、機械的破砕技術(ダウンス、ポリトロン、乳鉢と乳棒によるホモジナイズなど)を含む細胞溶解とタンパク質抽出法の概要。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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