71077

X-Gal Solution - Novagen

Convenience for blue/white screening

• 別名: X-Gal solution
• UNSPSCコード: 12352200
• NACRES: NA.54

特性

• 品質水準: 100
• フォーム: solution
• メーカー／製品名: Novagen^®
• 保管条件: OK to freeze
• テクニック: tissue culture: suitable
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

X-Gal, or 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, is an organic compound commonly used in molecular biology as a chromogenic substrate for the enzyme β-galactosidase, which is encoded by the LacZ gene. When β-galactosidase hydrolyzes X-Gal, it forms galactose and 5,5′-dibromo-4,4′-dichloro-indigo, resulting in the formation of intense blue color colonies. This process is widely used in LacZ activity assays and staining techniques, such as X-gal staining or LacZ staining to distinguish between recombinants (white) and nonrecombinants (blue). X-Gal staining is frequently used in the fields of molecular biology, genetics, and developmental biology to study gene expression and cell line tracing. X-Gal Solution is provided as a convenient 40 mg/ml concentrate in DMSO, ready for dilution into the culture medium or appropriate buffers.

アプリケーション

X-Gal Solution - Novagen has been used at 1:40 dilution to detect the expression of β-galactosidase and mutant fusion protein generated by the Apc^Δ242 mutation in the tissue sample.

警告

Toxicity: Irritant (B)

法的情報

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): 188.6 °F
• 引火点(℃): 87 °C