5.08226

Calyculin A, Discodermia calyx

InSolution, ≥98%, 100 µM in DMSO, Cell-permeable protein phosphatase 2A (PP2A) inhibitor

• 別名: InSolution Calyculin A, Discodermia calyx
• 実験式（ヒル表記法）: C₅₀H₈₁N₄O₁₅P
• CAS番号: 101932-71-2
• 分子量: 1009.17
• MDL番号: MFCD06795864
• UNSPSCコード: 12352200
• NACRES: NA.77

特性

• 品質水準: 100
• アッセイ: ≥98% (HPLC)
• 形状: liquid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• 保管温度: −70°C
• InChI: 1S/C50H81N4O15P/c1-29(20-22-51)16-14-17-30(2)32(4)24-33(5)42(57)35(7)38(55)25-41(65-13)45-46(69-70(61,62)63)49(8,9)50(68-45)26-39(56)34(6)40(67-50)19-15-18-36-27-66-48(53-36)31(3)21-23-52-47(60)44(59)43(58)37(28-64-12)54(10)11/h14-18,20,24,27,31,33-35,37-46,55-59H,19,21,23,25-26,28H2,1-13H3,(H,52,60)(H2,61,62,63)/b16-14+,18-15+,29-20-,30-17+,32-24+/t31-,33+,34+,35+,37-,38-,39-,40+,41-,42+,43-,44-,45+,46-,50-/m0/s1
• InChI Key: FKAWLXNLHHIHLA-QJLNTFFJSA-N

詳細

詳細

Cell-permeable protein phosphatase 2A (PP2A) inhibitor (IC₅₀ = 0.5-1.0 nM). Also inhibits protein phosphatase 1 (PP1; IC₅₀ = 2 nM). A non-phorbol ester type tumor promoter that induces oxidative DNA damage. Selectively increases the efficacy of AMPA receptor-mediated synaptic transmission at excitatory synapses. Recently, it has been used to study the regulation of Ca²⁺-activated K⁺ channels in smooth muscle and to correlate shape changes in fibroblasts with the degree of protein phosphorylation. Also known to prevent γ-radiation induced apoptosis in Burkitt′s lymphoma cell line BM13674.

生物化学的／生理学的作用

Cell permeable: yes

Primary Target
protein phosphatase-2A & -1

Reversible: yes

Target IC₅₀: 0.5-1.0 and 2 nM for PP2A and PP1, respectively

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

物理的形状

A 100 µM (5 µg/50 µL) sterile-filtered solution of Calyculin A, Discodermia calyx (Cat. No. 208851) in DMSO.

再構成

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C. Use only fresh DMSO for further dilutions.

その他情報

Murakami, N., et al. 1994. Neurosci. Lett.176, 181.
Takeuchi, T., et al. 1994. Biochem. Biophys. Res. Commun.205, 1803.
Figurov, A., et al. 1993. Eur. J. Neurosci.5, 1083.
Song, Q., and Lavin, M.F. 1993. Biochem. Biophys. Res. Commun.190, 47.
Gopalakrishna, R., et al. 1992. Biochem. Biophys. Res. Commun.189, 950.
Carl, A., et al. 1991. Am. J. Physiol.261, C387.
Suganuma, M., et al. 1990. Cancer Res.50, 3521.
Cohen, P. 1989. Annu. Rev. Biochem.58, 453.
Cohen, P., et al. 1989. J. Biol. Chem.264, 21435.
Ishihara, H., et al. 1989. Biochem. Biophys. Res. Commun.159, 871.
Kato, Y., et al. 1988. J. Org. Chem.53, 3930.
Kato, Y., et al. 1986. J. Am. Chem. Soc.108, 2780.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): 188.6 °F - (refers to pure substance)
• 引火点(℃): 87 °C - (refers to pure substance)