362185

N-Glycosidase F, Elizabethkingia meningosepticum

• 別名: N-Glycosidase F, Elizabethkingia meningosepticum, Glycopeptidase F, PNGase F
• CAS番号: 83534-39-8
• Enzyme Commission number: 3.5.1.52 (BRENDA, IUBMB)
• MDL番号: MFCD00131222
• UNSPSCコード: 12352202
• NACRES: NA.54

特性

• 由来生物: bacterial (Elizabethkingia meningosepticum)
• 品質水準: 100
• 結合体: (N-linked)
• 形状: liquid
• 比活性: ≥20,000 units/mg protein; ≥4500 units/mL
• メーカー／製品名: Calbiochem^®
• 保管条件: do not freeze
• その他の活性: Proteases, none detected
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

詳細

Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.

Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1-3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.

物理的形状

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

その他情報

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable