33-047

PI 3-Kinase 3-step HTRF^® Assay

The PI 3-Kinase 3-Step HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format.

• 別名: 3-Step HTRF Assay, PI 3-Kinase Assay
• UNSPSCコード: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

特性

• 品質水準: 100
• 交差性: human, mouse
• メーカー／製品名: HTRF^®; Upstate^®
• テクニック: activity assay: suitable (kinase)
• NCBIアクセッション番号: NM_006218.2
• UniProtアクセッション番号: P42336
• 検出方法: fluorometric (HTRF)
• 輸送温度: dry ice
• 遺伝子情報: human ... PIK3CA(5290)

詳細

詳細

Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit α (PIK3CA) gene is mapped to human chromosome 3q26.32. This gene codes for the catalytic subunit p110α of class IA PI3-kinase. PI3K signaling pathways regulate various cellular activities and properties including proliferation, survival, apoptosis, motility, and morphology. Mutation or overexpression of the PIK3CA gene is implicated in the development of various human solid tumors including head and neck cancers or pharyngeal cancer and ovarian cancer.

アプリケーション

PI 3-Kinase 3-step HTRF^® Assay has been used to determine the in vitro inhibitory potencies of phosphatidylinositol 3-kinase (PI3K) δ inhibitors such as MSD-496486311, MSD126796721, idelalisib, and duvelisib across PI3K α, β, γ, and δ isoforms.

生物化学的／生理学的作用

Target Sub-Family: PI3K/PI4K

包装

Kit capacity: 10,000 wells

構成

4X Reaction Buffer

PIP2 (substrate, 1mM stock)

DM A Reaction Buffer

DM B Reaction Buffer

DM C Reaction Buffer

BPIP3

Stop Reagent

保管および安定性

12 months at -70°C

その他情報

Research Category
Apoptosis & Cancer
Research Sub Category
Oncology

法的情報

HTRF is a registered trademark of Cis Bio International

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H319
• 注意書き: P264 - P280 - P305 + P351 + P338 - P337 + P313
• 危険有害性の分類: Eye Irrit. 2
• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable