324715

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

• 別名: Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes
• CAS番号: 72561-05-8
• Enzyme Commission number: 3.4.21.50 (BRENDA, IUBMB)
• MDL番号: MFCD02253020
• UNSPSCコード: 12352202

特性

• グレード: Proteomics Grade
• 品質水準: 100
• アッセイ: ≥90% (SDS-PAGE)
• フォーム: lyophilized
• 比活性: ≥200 units/mg protein
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic)
• 輸送温度: ambient
• 保管温度: 2-8°C

詳細

詳細

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK.

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

警告

Toxicity: Harmful (C)

単位の定義

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.

物理的形状

Lyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.

再構成

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Reconstitute in 50 µl of distilled H₂O.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany