324708

Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

• 別名: Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi
• CAS番号: 9001-92-7
• Enzyme Commission number: 3.4.24.33 (BRENDA, IUBMB)
• MDL番号: MFCD00004464
• UNSPSCコード: 12352202
• NACRES: NA.54

特性

• グレード: Proteomics Grade
• 品質水準: 100
• アッセイ: ≥90% (SDS-PAGE)
• フォーム: lyophilized solid
• 比活性: ≥20,000 units/mg protein
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 色: white
• 溶解性: water: soluble
• 輸送温度: ambient
• 保管温度: 2-8°C

詳細

詳細

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins and the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK.

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

警告

Toxicity: Harmful (C)

単位の定義

One unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.

物理的形状

Lyophilized from 10 mM Tris-HCl, pH 7.5.

再構成

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

その他情報

Drapeau, G.R. 1980. J. Biol. Chem.255, 839.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS08,GHS07
• シグナルワード: Danger
• 危険有害性情報: H315,H319,H334,H335
• 注意書き: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• 危険有害性の分類: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• ターゲットの組織: Respiratory system
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1