251765

DAR-4M AM

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

• 別名: DAR-4M AM, Diaminorhodamine-4M AM
• 実験式（ヒル表記法）: C₂₈H₃₁IN₄O₅
• 分子量: 630.47
• UNSPSCコード: 12352202
• NACRES: NA.25

特性

• 品質水準: 100
• アッセイ: ≥98% (HPLC)
• 形状: solution
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: dark red
• 蛍光検出: λ_ex ~560 nm; λ_em ~575 nm
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH above 4.0. Excitation max: ~560 nm; emission max: ~575 nm.

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.

生物化学的／生理学的作用

Cell permeable: yes

Primary Target
Nitric oxide (NO) fluorescent indicator

Product does not compete with ATP.

Reversible: no

包装

Packaged under inert gas

警告

Toxicity: Irritant (B)

物理的形状

In DMSO.

再構成

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.

その他情報

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

法的情報

Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): 188.6 °F
• 引火点(℃): 87 °C