251520-M

DAF-FM DA

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM.

• 別名: DAF-FM DA, 3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate
• 実験式（ヒル表記法）: C₂₅H₁₈F₂N₂O₇
• CAS番号: 254109-22-3
• 分子量: 496.42
• MDL番号: MFCD16872050
• UNSPSCコード: 12352204
• NACRES: NA.25

特性

• 品質水準: 100
• アッセイ: ≥98% (HPLC)
• フォーム: solution
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: pale yellow
• 蛍光検出: λ_ex ~500 nm; λ_em ~515 nm
• 保管温度: −20°C
• SMILES記法: Fc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)F)C42OC(=O)c5c4ccc(c5N)NC
• InChI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• InChI Key: BEVHTVRRVVEMEF-UHFFFAOYSA-N

詳細

詳細

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Intracellularly releases DAF-FM (Cat. No. 251515) by the action of esterases. Reacts with NO, in the presence of O₂, resulting in a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence is reported to be stable above pH 5.8.

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. Releases DAF-FM (Cat. No. 251515) by the action of intracellular esterases. Reacts with NO, in the presence of oxygen, resulting in the formation of a triazolo-fluorescein analog (DAF-FM T) that exhibits about 160-fold greater fluorescence quantum efficiency. The fluorescence intensity is reported to be stable above pH 5.8. Excitation max.: ~500 nm; emission max.: ~515 nm.

生物化学的／生理学的作用

Primary Target
Nitric oxide (NO) fluorescent indicator

警告

Toxicity: Irritant (B)

物理的形状

A 5 mM solution in DMSO.

再構成

Following initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution; discard any unused portion.

その他情報

Kojima, H., et al. 2001. J. Neurochem.76, 1404.
Park. J.M., et al. 2001. Br. J. Pharmacol.132, 1876.
Itoh, Y., et al. 2000. Anal. Biochem.287, 203.
Kojima, H., et al. 1999. Angew Chem. Int. Ed.38, 3209.

法的情報

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• 引火点(℃): 87 °C - closed cup - (Dimethylsulfoxide)