182515

CK-666

≥95% (HPLC), solid, Arp2/3 complex inhibitor, Calbiochem

• 別名: Arp2/3 Complex Inhibitor I, CK-666, 2-Fluoro-N-(2-(2-Methyl-1H-indol-3-yl)ethyl)benzamide, CK-0944666, Actin Assembly Inhibitor XIII, Actin Assembly Inhibitor XIII, 2-Fluoro-N-(2-(2-Methyl-1H-indol-3-yl)ethyl)benzamide, CK-0944666
• 実験式（ヒル表記法）: C₁₈H₁₇FN₂O
• CAS番号: 442633-00-3
• 分子量: 296.34
• MDL番号: MFCD03036271
• UNSPSCコード: 12352200
• NACRES: NA.77

特性

• 製品名: Arp2/3 Complex Inhibitor I, CK-666, Arp2/3 Complex Inhibitor I, CK-666, CAS 442633-00-3, is a cell-permeable selective inhibitor of actin assembly mediated by actin-related protein Arp2/3 complex (IC50 = 4 uM in human).
• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• フォーム: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: off-white
• 溶解性: DMSO: 50 mg/mL
• 輸送温度: ambient
• 保管温度: 2-8°C
• SMILES記法: Fc1c(cccc1)C(=O)NCCc2c3c([nH]c2C)cccc3
• InChI: 1S/C18H17FN2O/c1-12-13(14-6-3-5-9-17(14)21-12)10-11-20-18(22)15-7-2-4-8-16(15)19/h2-9,21H,10-11H2,1H3,(H,20,22)
• InChI Key: UXRKUKRXVWJFER-UHFFFAOYSA-N

詳細

詳細

A cell-permeable indolyl-fluorobenzamide compound that selectively inhibits actin assembly mediated by actin-related protein Arp2/3 complex of human, bovine, fission yeast S. pombe, and budding yeast S. cerevisiae origin (IC₅₀ = 4, 17, 5, and 12 µM, respectively), without affecting S. pombe formin domain Cdc12(FH2)-mediated or the spontaneous actin polymerization. Shown to inhibit the actin filament "comet tails" formation around intracellular Listeria in infected SKOV3 cells (IC₅₀ = 7 µM) in a reversible manner. Structrual studies indicate that CK-666 targets a pocket formed between subdomain 4 of Arp2 and subdomain 1 of Arp3, preventing Arp2/3 from shifting into an active conformation upon N-WASP-VCA binding. CK-666 and CK-869 (Cat. No. 182516) exhibit different modes of binding, resulting in their different yeast cross-reactivities. CK-689 (Cat. No. 182517) can serve as a negative control.

包装

Packaged under inert gas

警告

Toxicity: Regulatory Review (Z)

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

その他情報

Nolen, B.J., et al. 2009. Nature460, 1031.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable