176908

Anthrax Protective Antigen, Bacillus anthracis, Nicked, Recombinant, B. anthracis

• 別名: PA ₆₃, PA ₆₃
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• フォーム: lyophilized
• 品質水準: 100
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 輸送温度: ambient
• 保管温度: 2-8°C

詳細

詳細

Recombinant, Bacillus anthracis anthrax protective antigen (nicked) expressed in a specialized strain of B. anthracis. PA₆₃ is the carboxyl-terminal 63-kDa fragment obtained by the proteolytic cleavage of the receptor-bound protective antigen (PA), one of the three proteins that comprise anthrax toxin. Unlike native PA, PA₆₃ oligomerizes to form a ring-shaped heptamer that binds up to three copies of EF and/or LF competitively and with high affinity (K_d ~1 nM). Whereas native PA persists on the cell surface, the heptamer is endocytosed, presumably because oligomerization aggregates anthrax toxin receptor. The endocytosed toxic complexes are trafficked to endosomal compartments where the low pH causes the PA63 heptamer to insert into the membrane and form a water-filled channel.

Recombinant, Bacillus anthracis anthrax protective antigen(nicked) expressed in a special strain of B. anthracis. PA₆₃ is the carboxyl-terminal 63-kDa fragment obtained by the proteolytic cleavage of the receptor-bound protective antigen (PA), one of the three proteins that comprise anthrax toxin. Unlike native PA, PA₆₃ oligomerizes to form a ring-shaped heptamer that binds up to three copies of EF and/or LF competitively and with high affinity (K_d ~1 nM). Whereas native PA persists on the cell surface, the heptamer is endocytosed, presumably because oligomerization aggregates anthrax toxin receptor. The endocytosed toxic complexes are trafficked to endosomal compartments where the low pH causes the PA63 heptamer to insert into the membrane and form a water-filled channel.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
EF and/or LF

Product does not compete with ATP.

Reversible: no

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from 100 mM NaCL, 10 mM BIS-Tris propane, 1.25% trehalose, pH 8.5.

再構成

Following reconstitution aliquot and freeze (-20°C or- 70°C) for long term storage or refrigerate (4°C) for short-term (few hours) storage. Avoid rapid freeze-thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C.

Reconstitute in sterile 50% glycerol or sterile, distilled H₂O to a final concentration of 1 mg/ml. Addition of BSA to a concentration of 1 mg/ml may enhance stability.

アナリシスノート

Single major band at 63 kD by SDS-PAGE

その他情報

Mogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7045.
Nassi, S., et al. 2002. Biochemistry41, 1445.
Singh, Y., et al. 1999. Infect. Immun.67, 1853.
Leppla, S.H. 1988. Methods Enzymol.165, 103.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable