176905

Anthrax Protective Antigen, Bacillus anthracis, Recombinant, B. anthracis

Anthrax Protective Antigen, Bacillus anthracis, Recombinant, is the protective antigen that mediates the entry of anthrax lethal factor and edema factor into the target cell.

• 別名: Anthrax PA
• UNSPSCコード: 12352202

特性

• 品質水準: 100
• 形状: lyophilized
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 輸送温度: ambient
• 保管温度: 2-8°C

詳細

詳細

Recombinant, Bacillus anthracis anthrax protective antigen expressed in a special strain of B. anthracis. One of three protein components of anthrax toxin produced by the pathogenic bacterium, Bacillus anthracis. Central moiety that mediates entry of lethal factor (LF) and edema factor (EF) into the target cell. PA83 binds to the cell surface via a type I membrane receptor with a von Willebrand factor A domain, known as anthrax toxin receptor. PA83 is proteolytically activated in vivo by a furin-like protease to produce a 63 kDa protein (PA63). PA63 self-associates to form a heptameric, cation-selective, voltage-gated, membrane channel that subsequently binds to LF and/or EF. Cleavage of the 20 kDa fragment from PA83 is necessary for binding of protective antigen to LF and EF.

Recombinant, Bacillus anthracis anthrax protective antigen expressed in a specialized strain of B. anthracis. One of the three protein components of Anthrax toxin, protective antigen (PA) is the central moiety that mediates the entry of lethal factor and edema factor into the target cell. PA binds to the cell surface via a type I membrane protein with a von Willebrand factor A domain called anthrax toxin receptor. After proteolytic activation to a 63 kDa fragment, PA self-associates to form a heptameric cation-selective, voltage-gated membrane channel.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
Type I membrane protein with a von Willebrand factor A domain called anthrax toxin receptor

Product does not compete with ATP.

Reversible: no

包装

yes

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from 50 mM NaCl, 5 mM HEPES, pH 7.5.

再構成

Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 6 months at -20°C.

Reconstitute in 100 µl sterile, distilled H₂O to yield a final concentration of 1 mg/ml.

アナリシスノート

Single major band by SDS-PAGE. Several smaller minor components are apparent that may represent cleavage fragments of the PA.

その他情報

Mogridge, J., et al. 2002. Biochemistry41, 1079.
Nassi, S., et al. 2002. Biochemistry41, 1445.
Ahuja, N., et al. 2001. Biochem. Biophys. Res. Commun.286, 6.
Bradley, K.A., et al. 2001. Nature414, 225.
Brossier, F., and Mock, M. 2001. Toxicon39, 1747.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable