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由来生物
mouse
品質水準
抗体製品タイプ
primary antibodies
クローン
4G10®, monoclonal
化学種の反応性（ホモロジーによる予測）
all
メーカー／製品名
Upstate®
テクニック
immunoprecipitation (IP): suitable
アイソタイプ
IgG2bκ
輸送温度
wet ice
ターゲットの翻訳後修飾
phosphorylation (pTyr)
遺伝子情報
human ... PID1(55022)
詳細
The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. Now Millipore has made the best even better with 4G10 Platinum. We pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20, to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone is capable.
免疫原
The immunogen for 4G10 was phospho-tyramine coupled to KLH, while the immunogen for PY20 was phosphotyrosine conjugated to a carrier protein.
アプリケーション
4G10 Platinum, Anti-Phosphotyrosine, Agarose Conjugate detects levels of Phosphotyrosine proteins & has been published & validated for use in IP.
特徴および利点
Format: Gel Immobilized
品質
Immunoprecipitation:
Twenty μl of 50% slurry of this reagent (10 μl packed beads) was used to immunoprecipitate tyrosine-phosphorylated proteins from 250 μg PDGF-stimulated NIH3T3 cell lysate or EGF-stimulated A431 cell lysate.
Twenty μl of 50% slurry of this reagent (10 μl packed beads) was used to immunoprecipitate tyrosine-phosphorylated proteins from 250 μg PDGF-stimulated NIH3T3 cell lysate or EGF-stimulated A431 cell lysate.
ターゲットの説明
Dependent upon the molecular weight of the Tyrosine-phosphorylated protein
物理的形状
protein G purified mouse monoclonal 4G10 (IgG2bκ) and protein A purified PY20 (IgG2b)
Supplied as a 50% slurry of a proprietary mixture of agarose conjugated, protein G purified mouse monoclonal 4G10 (IgG2bκ) and protein A purified PY20 (IgG2b) in PBS, pH 7.4 with 0.05% sodium azide.
保管および安定性
Stable for 6 months at 2-8°C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
NOTE: DO NOT FREEZE.
NOTE: DO NOT FREEZE.
法的情報
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
16-638:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
S Ruff-Jamison et al.
The Journal of biological chemistry, 266(10), 6607-6613 (1991-04-15)
Phosphotyrosine and similar analogs have been used to elicit antibodies that have found widespread use in the study of cellular tyrosine phosphorylation. In order to better understand the anti-phosphotyrosine immune response and to elucidate the details of the specific association
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