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由来生物
mouse
品質水準
結合体
FITC conjugate
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
JBW301, monoclonal
交差性
human
メーカー／製品名
Upstate®
テクニック
flow cytometry: suitable
immunocytochemistry: suitable
アイソタイプ
IgG1
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
phosphorylation (pSer139)
遺伝子情報
human ... H2AX(3014)
詳細
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
特異性
Broad species cross-reactivity is expected based on conservation of sequence homology.
Recognizes Histone H2A.X phosphorylated at Ser139, MW 15 kDa.
免疫原
Epitope: Ser139
KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse. Clone JBW301.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン
ヒストン
Immunocytochemistry: 2-4 μg/mL of a previous lot detected phospho-histone H2A.X in etoposide-treated HeLa cells fixed with 95% ethanol/5% acetic acid (10 minutes), followed by 1% formaldehyde, 0.25% Triton™ X-100 in TBS (5 min.).
Use Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate (mouse monoclonal antibody) validated in FC, ICC to detect phospho-Histone H2A.X (Ser139) also known as H2AXS139P, H2AX histone.
品質
Evaluated by Flow Cytometry to detect Log phase Jurkat cells treated with staurosporine.
Flow Cytometry: Log phase Jurkat cells were treated with staurosporine (1 μg/mL) for the indicated time. Histone H2A.X (Ser 139) phosphorylation was detected as described in the manual for the H2A.X Phosphorylation Assay Kit (Flow Cytometry), Catalog # 17-344. Cells were analyzed on a Becton-Dickinson FACS-Calibur flow cytometer.
Flow Cytometry: Log phase Jurkat cells were treated with staurosporine (1 μg/mL) for the indicated time. Histone H2A.X (Ser 139) phosphorylation was detected as described in the manual for the H2A.X Phosphorylation Assay Kit (Flow Cytometry), Catalog # 17-344. Cells were analyzed on a Becton-Dickinson FACS-Calibur flow cytometer.
ターゲットの説明
15 kDa
物理的形状
Protein G Purified
Purified FITC-conjugated mouse monoclonal IgG1 in buffer containing PBS with 0.05% sodium azide. Frozen solution.
保管および安定性
Stable for 1 year at from date of receipt.
アナリシスノート
Control
Staurosporine-treated Jurkat cells
Staurosporine-treated Jurkat cells
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
16-202A:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Validation of a flow cytometry based G(2)M delay cell cycle assay for use in evaluating the pharmacodynamic response to Aurora A inhibition.
Estevam J, Danaee H, Liu R, Ecsedy J, Trepicchio WL, Wyant T
Journal of Immunological Methods null
Role of transcriptional corepressor CtBP1 in prostate cancer progression.
Wang, R; Asangani, IA; Chakravarthi, BV; Ateeq, B; Lonigro, RJ; Cao, Q; Mani, RS; Camacho et al.
Neoplasia null
David W Schoppy et al.
The Journal of clinical investigation, 122(1), 241-252 (2011-12-03)
Oncogenic Ras and p53 loss-of-function mutations are common in many advanced sporadic malignancies and together predict a limited responsiveness to conventional chemotherapy. Notably, studies in cultured cells have indicated that each of these genetic alterations creates a selective sensitivity to
Protective role of Puralpha to cisplatin.
Kaminski, R; Darbinyan, A; Merabova, N; Deshmane, SL; White, MK; Khalili, K
Cancer Biology & Therapy null
Gene deregulation and spatial genome reorganization near breakpoints prior to formation of translocations in anaplastic large cell lymphoma.
Mathas, S; Kreher, S; Meaburn, KJ; Johrens, K; Lamprecht, B; Assaf, C; Sterry, W; Kadin et al.
Proceedings of the National Academy of Sciences of the USA null
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