おすすめの製品
由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
4G10®, monoclonal
化学種の反応性
vertebrates
メーカー／製品名
Upstate®
テクニック
immunoprecipitation (IP): suitable
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... PID1(55022)
詳細
Anti-Phosphotyrosine antibody (05-321), cross-linked to protein A agarose by dimethylpimelimidate
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
特異性
Recognizes tyrosine-phosphorylated proteins from all species.
免疫原
Phosphotyramine-KLH.
アプリケーション
Research Category
細胞シグナル伝達
細胞シグナル伝達
Research Sub Category
一般的な翻訳後修飾
一般的な翻訳後修飾
Anti-Phosphotyrosine Antibody, clone 4G10, agarose conjugate is an antibody against Phosphotyrosine for use in IP.
This product is useful for immunoprecipitation and immunoaffinity chromatography methods.
Suitable for immunoprecipitation and affinity purification.
Suitable for immunoprecipitation and affinity purification.
特徴および利点
Format: Gel Immobilized
品質
Evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.
Immunoprecipitation Analysis:
10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
Immunoprecipitation Analysis:
10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
ターゲットの説明
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
物理的形状
Protein A purified
1 mg of Anti-Phosphotyrosine monoclonal antibody covalently coupled to 1 mL of protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.05% Kathon as a preservative, for a total volume of 2 mL. Liquid suspension.
保管および安定性
Stable for 1 year at 2-8ºC from date of receipt.
アナリシスノート
Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
10 - Combustible liquids
WGK
WGK 1
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Identification of oncogenic point mutations and hyperphosphorylation of anaplastic lymphoma kinase in lung cancer.
Wang, YW; Tu, PH; Lin, KT; Lin, SC; Ko, JY; Jou, YS
Neoplasia null
Activation of src-related tyrosine kinases by IL-3
Anderson, S. M. and Jorgensen, B.
Journal of immunology (Baltimore, Md. : 1950), 155, 1660-1670 (1995)
Robin White et al.
The Journal of biological chemistry, 287(3), 1742-1754 (2011-12-01)
Myelin basic protein (MBP) is a major component of central nervous system (CNS) myelin. The absence of MBP results in the loss of almost all compact myelin in the CNS. MBP mRNA is sorted into RNA granules that are transported
Heparin binding and oligomerization of hepatocyte growth factor/scatter factor isoforms. Heparan sulfate glycosaminoglycan requirement for Met binding and signaling.
Sakata, H, et al.
The Journal of Biological Chemistry, 272, 9457-9463 (1997)
Phosphorylation site dynamics of early T-cell receptor signaling.
Chylek, LA; Akimov, V; Dengjel, J; Rigbolt, KT; Hu, B; Hlavacek, WS; Blagoev, B
Testing null
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）