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Merck

1.11277

Millipore

m-ENDO agar LES

GranuCult® plus, APHA, suitable for microbiology, for coliforms

別名:

Coliform selective agar, modified Endo medium

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About This Item

UNSPSCコード:
41106213
NACRES:
NA.74

認証

APHA

品質水準

無菌性

non-sterile

形状

granular

包装

pkg of 500 g

メーカー/製品名

GranuCult® plus

保管条件

(Tightly closed. Dry. Keep in a well-ventilated place.)

テクニック

microbiological culture: suitable

dark violet

pH

7.0-7.4 (25 °C in H2O, ready-to-use solution; after autoclaving)

溶解性

51 g/L

アプリケーション

food and beverages
microbiology

保管温度

15-25°C

適合性

coliforms

詳細

m-ENDO Agar LES (Lawrence Experimental Station) is a medium recommended for the enumeration of coliforms in water used in the Standard Total Coliform Membrane Filter Procedure. The American Public Health Association (APHA) recommends m-Endo Agar in standard total coliform membrane filtration for testing potable water and wastewater. It follows the two-step membrane filter procedure using Lauryl Sulfate Broth as a preliminary enrichment, resulting in higher coliform counts. Peptones in the medium are a source of carbon, nitrogen, vitamins, and minerals. Yeast extract supplies B-complex vitamins, which stimulate bacterial growth. Lactose is a carbohydrate. Phosphates are buffering agents. Sodium chloride maintains the osmotic balance of the medium. Sodium desoxycholate and sodium lauryl sulfate are added to inhibit the growth of the accompanying flora. Pararosaniline (fuchsin) is an indicator. Sodium sulfite is added to decolorize the basic fuchsin solution. Agar is the solidifying agent. Lactose-positive colonies are colored red due to the liberation of fuchsin from the fuchsin-sulfite compound. E. coli colonies develop a metallic sheen due to the production of aldehydes with the rapid fermentation of lactose. Lactose-nonfermenting bacteria appear as clear, colorless colonies.

アプリケーション

m-ENDO agar LES is suitable for the enumeration of coliforms in water using a two-step membrane filtration technique.

特徴および利点

  • GranuCult® offers superior granulated culture media
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

アナリシスノート

Appearance (clearness): clear to turbid
Appearance (color): red
pH-value (25 °C): 7.0 - 7.4
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Enterobacter aerogenes ATCC 13048): good to very good
Growth (Proteus mirabilis ATCC 14273): good to very good
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): none
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): none to poor
Colony color (Escherichia coli ATCC 25922 (WDCM 00013)): red
Colony color (Enterobacter aerogenes ATCC 13048): red
Colony color (Proteus mirabilis ATCC 14273): reddish/red
Metallic luster (Escherichia coli ATCC 25922 (WDCM 00013)): +
Metallic luster (Enterobacter aerogenes ATCC 13048): +
Metallic luster (Proteus mirabilis ATCC 14273): -
Incubation: 24 hrs.; 35 °C; aerobic.

脚注

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法的情報

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

ピクトグラム

Health hazard

シグナルワード

Danger

危険有害性情報

危険有害性の分類

Carc. 1B

保管分類コード

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

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