100081

14-3-3 Antagonist I, 2-5

The 14-3-3 Antagonist I, 2-5 controls the biological activity of 14-3-3.

• 別名: 14-3-3 Antagonist I, 2-5, (S)-2-(2-(4-Azidobenzamido)acetamido)-3-((4-hydroxyphenethyl)amino)-3-oxopropyl-dihydrogen phosphate
• 実験式（ヒル表記法）: C₂₀H₂₃N₆O₈P
• 分子量: 506.41
• UNSPSCコード: 12352200

特性

• 品質水準: 100
• アッセイ: ≥90% (HPLC)
• フォーム: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic); protect from light
• 色: pale yellow to beige
• 溶解性: DMSO: 50 mg/mL
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

A cell-permeable dipeptidyl-phosphoserine compound that targets 14-3-3 phosphopeptide binding site and effectively competes against 14-3-3 ligands, including Raf-1 and p53, binding to 14-3-3 (IC₅₀ = 2.6 µM against TMR-GG-RLSHpSSLPG binding to human 14-3-3ς). Shown to induce caspase-3 activation and apoptotic cell death in human lung adenocarcinoma A549 cultures (live cell population after 12 h 100 µM drug treatment = 30% of control). Stability study using A549 cell lysates (100 µM drug in 1 µg/ml lysate) reveals a dephosphorylation t_1/2 of ~ 6 hrs and no detectable proteolytic degradation up to 12 hrs.

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

その他情報

Wu, H., et al. 2010. Angew. Chem. Int. Ed.49, 6528.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable