1.00079

Bolton Broth Selective Supplement

according to ISO 10272-1, for Campylobacter spp., pkg of 10 vials, For the preparation of Bolton Selective Enrichment Broth

• 別名: Campylobacter Selective Supplement
• UNSPSCコード: 41106212
• NACRES: NA.74

特性

• 認証: according to ISO 10272-1
• 品質水準: 200
• 法規制遵守: FDA -BAM
• 形状: lyophilized powder
• 組成: Amphotericin B, 5 mg ; Cefoperazone, 10 mg ; Trimethoprim, 10 mg ; Vancomycin, 10 mg
• 包装: pkg of 10 vials
• テクニック: microbiological culture: suitable
• アプリケーション: food and beverages; microbiology; pathogen testing
• 保管温度: 2-8°C
• 適合性: Campylobacter spp.; Listeria spp.

詳細

詳細

Bolton Broth Selective Supplement is a mixture of four different antibiotics in lyophilized form. Vancomycin, Cefoperazone, and Trimethoprim inhibit the growth of Gram-positive and Gram-negative bacteria. Amphotericin B largely reduces the growth of Yeasts and Molds.

アプリケーション

Bolton Broth Selective Supplement is an additive used during the preparation of Bolton Selective Enrichment Broth for the enrichment of Campylobacter from foods.

関連事項

Replaces: 1.00079.0017

アナリシスノート

Growth promotion test according to ISO 11133
Mixed inoculum of Campylobacter, E.coli and P.mirabilis.
Additionaly monoculture of E.coli and P.mirabilis.
Afterwards confirmation on mCCDA resp. Tryptic soy agar
Inoculum on reference medium
- Campylobacter coli ATCC 43478 (WDCM 00004):
10 - 100
- Campylobacter jejuni ATCC 33291 (WDCM 00005): 10 - 100
- Campylobacter jejuni ATCC 29428 (WDCM 00156): 10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013): ≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012): ≥ 1000
- Proteus mirabilis ATCC 29906 (WDCM 00023): ≥ 1000
Growth on mCCDA
- Campylobacter coli ATCC 43478 (WDCM 00004):
>10 CFU
- Campylobacter jejuni ATCC 33291 (WDCM 00005): >10 CFU
- Campylobacter jejuni ATCC 29428 (WDCM 00156): >10 CFU
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Proteus mirabilis ATCC 29906 (WDCM 00023): no growth
Colony colour
- Campylobacter coli ATCC 43478 (WDCM 00004):
greyish
- Campylobacter jejuni ATCC 33291 (WDCM 00005): greyish
- Campylobacter jejuni ATCC 29428 (WDCM 00156): greyish
Incubation:
Bolton broth: 5 hours at 44°C, afterwards 44 hours at 41.5°C, Anaerocult C
Confirmation on mCCDA: at least 44 hours at 41.5°C, Anaerocult C
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H317 - H334 - H361d - H373
• 注意書き: P201 - P202 - P280 - P302 + P352 - P304 + P340 + P312 - P308 + P313
• 危険有害性の分類: Repr. 2 - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 2
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3