07-131-AF488

Anti-Sirt1 (Sir2) Antibody, Alexa Fluor^™ 488 Conjugate

from rabbit, ALEXA FLUOR^™ 488

• 別名: NAD-dependent protein deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, SIR2alpha, Sir2, mSIR2a, SirtT1 75 kDa fragment, 75SirT1, Sirt1(Sir2), Alexa Fluor^™ 488 Conjugate
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: ALEXA FLUOR^™ 488
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: mouse, human
• テクニック: immunocytochemistry: suitable
• NCBIアクセッション番号: NP_062786
• UniProtアクセッション番号: Q923E4
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SIRT1(23411)

詳細

詳細

NAD-dependent protein deacetylase sirtuin-1 (UniProt Q923E4; also known as Sir2, mSIRT2a, NAD-dependent deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, Sir2-like 1, SIR2-like protein 1, SIR2alpha) is encoded by the Sirt1 (also known as Sir2l1) gene (Gene ID 93759) in murine species. Sirtuins constitute a family of NAD+-dependent deacetylases found in nearly all organisms. Sirtuins have been implicated in the regulation of aging, transcription, apoptosis, and stress resistance. Full-length SirT1 (FLSirT1) plays an important role in human cartilage homeostasis and chondrocyte survival by mediating the expression of major cartilage anabolic components, collagen 2(I) & aggrecan. The generation of the transcriptionally inactive 75-kDa fragment (75SirT1) via site-specific cleavage of FLSirT1 at amino acid 533 by cathepsin B is an anti-apoptotic mechanism to promote human osteoarthritic (OA) chondrocytes survival following exposure to proinflammatory cytokines. The 5SirT1 fragment is shown to associate with cytochrome c and likely blocks downstream apoptosome assembly. Phosphorylation plays an important role in modulating SirT1 activity and cellular functions. Multiple kinases and their corresponding SirT1 phosphorylation sites have been identified, including pT344 targeted by AMPK, pT530 and pS540 targeted by Cdk1/Cyclin B, pS46 (mouse) or pS47 (human) targeted by JNK1, pT522 targeted by DYRK1A & DYRK3, as well as pS27 & pS47 targeted by CaMKKβ.

特異性

This antibody recognizes Sirt1.

免疫原

Epitope: a.a. 1-131

GST-tagged fusion protein corresponding to amino acids 1-131 of mouse Sir2α.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
核受容体

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Sirt1(Sir2) in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-131) is shown to be suitable also for Western blotting, immunoprecipitation, and ChIP applications.

This Anti-Sirt1 (Sir2) Antibody, Alexa Fluor^™ 488 Conjugate is validated for use in Immunocytochemistry for the detection of Sirt1 (Sir2).

品質

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Sirt1(Sir2) in A431 cells.

ターゲットの説明

~110 kDa observed

物理的形状

Affinity purified

Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

法的情報

ALEXA FLUOR is a trademark of Life Technologies

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable