07-1238

Anti-phospho-MARCKS (Ser152/156) Antibody

from rabbit, purified by affinity chromatography

• 別名: Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate, 80 kDa protein, light chain
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse, rat, human
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NM_002356.5
• UniProtアクセッション番号: P29966
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pSer152/pSer156)
• 遺伝子情報: human ... MARCKS(4082)

詳細

詳細

Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a widely expressed substrate of PKC. MARCKS is essential in neuronal development, as mice deficient in it die shortly after birth. Through its basic effector region, MARCKS associates with acidic membrane phospholipids, actin filaments, and calmodulin. Poly (ADP-ribose) also binds the basic effector region, blocking calmodulin binding, membrane association, and phosphorylation by PKC, suggesting that MARCKS may be an effector of the DNA-damage signal transduced by poly (ADP-ribose).

特異性

MARCKS, phosphoSer152/156.

免疫原

Epitope: phosphoSer 152/156

Synthetic peptide corresponding to amino acids surrounding the phosphoSer152/156 of rat MARCKS.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
細胞骨格シグナル伝達

Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining of MARCKS With Citrate pH 6.0 Epitope Retrieval: Rat Placenta

Optimal working dilutions must be determined by the end user.

This Anti-phospho-MARCKS (Ser152/156) Antibody is validated for use in WB, IH(P) for the detection of phospho-MARCKS (Ser152/156).

品質

Routinely evaluated by Western Blot on EGF treated A431 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected phosphorylated MARCKS on 10 μg of EGF treated A431 lysates.

ターゲットの説明

~80 kDa

関連事項

Replaces: AB5879

物理的形状

Affinity purified

Purified rabbit polyclonal IgG in 10 mM Hepes, pH 7.5, with 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.

保管および安定性

Stable at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

アナリシスノート

Control
A431 Cell Lysate

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2