由来生物
rabbit
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
Tetrahymena sp., human
メーカー／製品名
Upstate®
濃度
1 mg/mL
テクニック
ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
acetylation (Lys5,Lys8,Lys 12,Lys16)
遺伝子情報
human ... H4C1(8359)
詳細
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
特異性
Other species not tested.
Recognizes acetylated histone H4.
Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May crossreact with other acetylated proteins.
Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May crossreact with other acetylated proteins.
免疫原
Epitope: a.a. 2-19
KLH-conjugated peptide corresponding to amino acids 2-19 of Tetrahymena histone H4 (AGG(Ac)KGG(Ac)KGMG(Ac)KVGA(Ac)KRHS-C), acetylated on lysines 5,8,12 and 16.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン
ヒストン
Anti-acetyl-Histone H4 Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a & has been validated in ChIP, ICC, IP & WB.
Immunoprecipitation:
5 μg of a previous lot immunoprecipitated acetylated histone H4 from 500 μg of acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate for 24 hours.
Immunocytochemistry:
5-10 μg/mL of a previous lot lot gave positive nuclear immunostaining in human A431 cells treated with sodium butyrate and fixed with ice-cold 1% paraformaldehyde in PBS.
Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate acetylated histone H4 from chromatin.
5 μg of a previous lot immunoprecipitated acetylated histone H4 from 500 μg of acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate for 24 hours.
Immunocytochemistry:
5-10 μg/mL of a previous lot lot gave positive nuclear immunostaining in human A431 cells treated with sodium butyrate and fixed with ice-cold 1% paraformaldehyde in PBS.
Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate acetylated histone H4 from chromatin.
品質
Routinely evaluated by western blot on acid-extracted proteins from HeLa cells treated with sodium butyrate.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected acetylated histone H4 in acid-extracted proteins from HeLa cells treated with 5 mM sodium butyrate. A previous lot detected acetylated histone H4 in Tetrahymena macronuclei. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected acetylated histone H4 in acid-extracted proteins from HeLa cells treated with 5 mM sodium butyrate. A previous lot detected acetylated histone H4 in Tetrahymena macronuclei. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.
ターゲットの説明
10 kDa
物理的形状
Protein A chromatography
Format: Purified
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide.
保管および安定性
1 year at -20°C from date of shipment.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
アナリシスノート
Control
TSA-treated NIH/3T3, A431, L929 (mouse) and C6 (rat) cell extracts, human breast carcinoma.
TSA-treated NIH/3T3, A431, L929 (mouse) and C6 (rat) cell extracts, human breast carcinoma.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
06-598:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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