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由来生物
rabbit
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
Apu2, monoclonal
化学種の反応性
rabbit, mouse, human, rat
テクニック
cell based assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... UBAP2(55833)
関連するカテゴリー
詳細
DI-UBIQUITIN PROTEIN AVAILABLE!
Di-Ubiquitin (Lys48-linked) Recombinant Protein (17-10408)
Di-Ubiquitin (Lys48-linked) Recombinant Protein (17-10408)
Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.
In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
特異性
Based on sequence homology, broad species cross-reactivity is expected.
Recognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin.
免疫原
Epitope: Lys48 ubiquitination site
Immunogen used was human di-ubiquitin linked at Lys48.
アプリケーション
Research Category
細胞シグナル伝達
細胞シグナル伝達
Research Sub Category
ユビキチン及びユビキチン代謝
ユビキチン及びユビキチン代謝
Immunohistochemistry:
Ubiquitin (Lys 48-specific) staining of rat brain sections (left figure) or human breast carcinoma (right figure). Staining pattern detects axons of neurons in brain tissue but not surrounding glial cells, and breast tumor cell cytoplasm is strongly detected without nuclear involvement.
Immunocytochemistry:
Confocal IF analysis of NIH/3T3, HeLa, and A431 cells using anti-Ubiquitin (Lys48 specific) (Red). Actin filaments were labeled with AlexaFluor 488-Phalloidin (Green). Nuclear material is stained with DAPI (Blue).
Flow cytometry:
Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys48-specific (#05-1307) (blue) and rabbit IgG control (red).
Immunoprecipitation:
Rabbit monoclonal anti-ubiquitin (Lys-48-specific), clone Apu2, precipitated ubiquitin-containing proteins from HeLa cell lysates.
Inhibition Assay:
Preincubation of anti-ubiquitin, Lys48-specific, with K48-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.
Ubiquitin (Lys 48-specific) staining of rat brain sections (left figure) or human breast carcinoma (right figure). Staining pattern detects axons of neurons in brain tissue but not surrounding glial cells, and breast tumor cell cytoplasm is strongly detected without nuclear involvement.
Immunocytochemistry:
Confocal IF analysis of NIH/3T3, HeLa, and A431 cells using anti-Ubiquitin (Lys48 specific) (Red). Actin filaments were labeled with AlexaFluor 488-Phalloidin (Green). Nuclear material is stained with DAPI (Blue).
Flow cytometry:
Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys48-specific (#05-1307) (blue) and rabbit IgG control (red).
Immunoprecipitation:
Rabbit monoclonal anti-ubiquitin (Lys-48-specific), clone Apu2, precipitated ubiquitin-containing proteins from HeLa cell lysates.
Inhibition Assay:
Preincubation of anti-ubiquitin, Lys48-specific, with K48-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.
Anti-Ubiquitin Antibody, Lys48-Specific, clone Apu2 is a high quality Rabbit Monoclonal Antibody for the detection of Ubiquitin & has been validated in FC, ICC, IP, IHC, WB, PIA.
品質
Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected Lys-48 ubiquitin chains by Western blotting.
A 1:1000-1:5000 dilution of this lot detected Lys-48 ubiquitin chains by Western blotting.
ターゲットの説明
varies
物理的形状
Protein A purified
Format: Purified
Protein A purified recombinant purified recombinant rabbit monoclonal antibody that consists of a humanized fAb fragment fused to a rabbit Fc tail produced in a CHO recombinant antibody system. Supplied in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
アナリシスノート
Control
Lys48 polyubiquitin chains.
Lys48 polyubiquitin chains.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
05-1307:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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