04-973

Anti-CD39 Antibody, clone 5F2

clone 5F2, from mouse

• 別名: ectonucleoside triphosphate diphosphohydrolase 1, ATPDase, ecto-apyrase, NTPDase-1, Ecto-ATP diphosphohydrolase, Lymphoid cell activation antigen, NTPDase 1, Ecto-apyrase, CD39 antigen
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 5F2, monoclonal
• application: FACS; WB
• 化学種の反応性: mouse
• テクニック: flow cytometry: suitable; western blot: suitable

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 5F2, monoclonal
• 化学種の反応性: mouse
• テクニック: flow cytometry: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_033978
• UniProtアクセッション番号: P55772
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Entpd1(12495)

詳細

詳細

Ectonucleoside triphosphate diphosphohydrolase 1 (NTDase 1 or CD39) belongs to the membrane-bound ecto-nucleoside triphosphates diphosphohydrolase family (E-NTPDases) that are characterized for their ability to hydrolyze nucleoside di- and triphosphates, such as ATP, in the presence of cations, i.e. CA2+ and Mg2+. CD39 is an important membrane protein containing two transmembrane domains and a large NTPDase active, extracellular region containing apyrase conserved regions. It is heavily glycosolated, having six known N-glycosolation sites, and functions on the plasma membrane’s outer surface. CD39 initiates the metabolism, of ATP and ADP to adenosine through a 2-step exzymatic reaction, providing extracellular adenosine for the purpose of regulating various inflammatory endpoints. It is a putative marker for B-cell activation and its expression has also been observed on NK-cells, dendritic cells, T-cells, and vascular endothelial cells. CD39 is a known inhibitor of ADP-induced platelet aggregation and may also play a role in angiogenesis.

特異性

This antibody recognizes CD39.

免疫原

Recombinant protein corresponding to mouse CD39.

アプリケーション

This Anti-CD39 Antibody, clone 5F2 is validated for use in FC, WB for the detection of CD39.

Western Blot Analysis: A previous lot was used by an independent laboratory in WB.

品質

Evaluated by Flow Cytometry in BALB/c splenocytes.

Flow Cytometry Analysis: 0.1 µg/mL of this antibody detected CD39 in BALB/c splenocytes.

ターゲットの説明

~ 100 kDa
It has been published that CD39 shows molecular weights between ~70-100 kDa (Wang, T.F. et al., 1996).

物理的形状

Format: Purified

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable